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Catalog Number Size Price
LS-B3423-200 200 µl (0.5 mg/ml) $460 
LIN28B Antibody - Anti-LIN28B antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
LIN28B Antibody - Anti-LIN28B antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
LIN28B Antibody - Fluorescent confocal image of SY5Y cells stained with LIN28B antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated LIN28B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the LIN28B immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000187772).
LIN28B Antibody - Western blot of LIN28B Antibody in HL60 cell line lysates (35 ug/lane). LIN28B (arrow) was detected using the purified antibody.
LIN28B Antibody - Flow cytometric of HL-60 cells using LIN28B Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
LIN28B Antibody - Anti-LIN28B antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
LIN28B Antibody - Anti-LIN28B antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
LIN28B Antibody - Fluorescent confocal image of SY5Y cells stained with LIN28B antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated LIN28B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the LIN28B immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000187772).
LIN28B Antibody - Western blot of LIN28B Antibody in HL60 cell line lysates (35 ug/lane). LIN28B (arrow) was detected using the purified antibody.
LIN28B Antibody - Flow cytometric of HL-60 cells using LIN28B Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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IHC‑plus™ Polyclonal Rabbit anti‑Human LIN28B Antibody (aa1‑30, IHC, IF, WB) LS‑B3423

IHC‑plus™ Polyclonal Rabbit anti‑Human LIN28B Antibody (aa1‑30, IHC, IF, WB) LS‑B3423

Note: This antibody replaces LS-C98240
Antibody:
LIN28B Rabbit anti-Human Polyclonal (aa1-30) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B3423-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
LIN28B Rabbit anti-Human Polyclonal (aa1-30) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
LIN28B antibody LS-B3423 is an unconjugated rabbit polyclonal antibody to human LIN28B (aa1-30). Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human LIN28B
Synonyms
LIN28B | CSDD2 | Lin-28B | Lin-28 homolog B (C. elegans) | Protein lin-28 homolog B | Lin-28.2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa1-30
Specificity
This LIN28B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LIN28B.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B3423 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3423 was determined to be 10 ug/ml.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LIN28B
Q6ZN17 NP_001004317.1

Validation

LIN28B Antibody - Anti-LIN28B antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-LIN28B antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-LIN28B antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LIN28B Antibody - Anti-LIN28B antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-LIN28B antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-LIN28B antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ LIN28B Antibody (aa1-30) for IHC, IF/Immunofluorescence, WB/Western, Flow LS-B3423 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)

Publications (2)

Lin28A and Lin28B inhibit let-7 microRNA biogenesis by distinct mechanisms. Piskounova E, Polytarchou C, Thornton JE, LaPierre RJ, Pothoulakis C, Hagan JP, Iliopoulos D, Gregory RI. Cell. 2011 Nov;147:1066-79.

Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression. Meiyan Qi , Yun Xia , Yanjun Wu, Zhuo Zhang, Xinyu Wang, Liying Lu, Cheng Dai, Yanan Song, Keying Xu, Weiwei Ji, Lixing Zhan. Nature communications. 2022 February;13:897.

Customer Reviews (0)


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Requested From: United States
Date Requested: 6/15/2024