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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B7681-50 50 µl (1 mg/ml) $425 
LIG1 / DNA Ligase 1 Antibody - Anti-DNA Ligase 1 / LIG1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
LIG1 / DNA Ligase 1 Antibody - Western blot analysis of lysates from HT-29 cells, using DNL1 Antibody. The lane on the right is blocked with the synthesized peptide.
LIG1 / DNA Ligase 1 Antibody - Anti-DNA Ligase 1 / LIG1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
LIG1 / DNA Ligase 1 Antibody - Western blot analysis of lysates from HT-29 cells, using DNL1 Antibody. The lane on the right is blocked with the synthesized peptide.
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IHCPlus™ Polyclonal Rabbit anti‑Human LIG1 / DNA Ligase 1 Antibody (aa111‑160, IHC, WB) LS‑B7681

IHCPlus™ Polyclonal Rabbit anti‑Human LIG1 / DNA Ligase 1 Antibody (aa111‑160, IHC, WB) LS‑B7681

Note: This antibody replaces LS-C119546
Antibody:
Rabbit Polyclonal to Human LIG1 / DNA Ligase 1
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B7681-50
50 µl
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human LIG1 / DNA Ligase 1
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
DNA Ligase 1 antibody LS-B7681 is an unconjugated rabbit polyclonal antibody to human DNA Ligase 1 (LIG1) (aa111-160). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human LIG1 / DNA Ligase 1
Synonyms
LIG1 | DNA ligase I | DNA ligase 1 | Ligase I, DNA, ATP-dependent
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from human DNL1. (AA Range: 111-160)
Epitope
aa111-160
Specificity
DNL1 Antibody detects endogenous levels of total DNL1 protein.
Usage
Western Blot: Predicted MW 101 kDa
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LIG1 / DNA Ligase 1
P18858 NM_000234 NP_000225.1

Validation

LIG1 / DNA Ligase 1 Antibody - Anti-DNA Ligase 1 / LIG1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Anti-DNA Ligase 1 / LIG1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Anti-DNA Ligase 1 / LIG1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.

LSBio Ratings

IHC-plus™ LIG1 / DNA Ligase 1 Antibody (aa111-160) for IHC, WB/Western LS-B7681 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 5/28/2020