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IHCPlus™ LGALS9 / Galectin 9 Antibody aa1‑323 Polyclonal ELISA,IHC,WB LS‑B13821

Catalog Number Size Price
LS-B13821-50 50 µg (1 mg/ml) $425 
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunofluorescence staining of NIH/3T3 cells with LGALS9 Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western blot of Galectin-9 antibody at 2 ug/ml with mouse heart tissue. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 40 KDa. Observed band size: 40 KDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot Positive WB detected in: Mouse heart tissue All lanes: LGALS9 antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40, 36, 35, 27, 26, 39 kDa Observed band size: 40 kDa
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunofluorescence staining of NIH/3T3 cells with LGALS9 Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western blot of Galectin-9 antibody at 2 ug/ml with mouse heart tissue. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 40 KDa. Observed band size: 40 KDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot Positive WB detected in: Mouse heart tissue All lanes: LGALS9 antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40, 36, 35, 27, 26, 39 kDa Observed band size: 40 kDa
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IHCPlus™ LGALS9 / Galectin 9 Antibody aa1‑323 Polyclonal ELISA,IHC,WB LS‑B13821

Note: This antibody replaces LS-C318560
Antibody:
Rabbit Polyclonal to Human LGALS9 / Galectin 9
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-B13821-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human LGALS9 / Galectin 9
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Galectin 9 antibody LS-B13821 is an unconjugated rabbit polyclonal antibody to human Galectin 9 (LGALS9 ). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human LGALS9 / Galectin 9
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, HRP, Biotin.
Purification
Caprylic acid and ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (7.5 µg/ml)
  • Western blot
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human Galectin-9 protein.
Epitope
aa1-323
Specificity
Human LGALS9 / Galectin 9
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LGALS9 / Galectin 9
O00182 NM_009587 NP_033665.1

Validation

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Requested From: United States
Date Requested: 9/18/2019