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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B5903-50 50 µg (0.5 mg/ml) $425 
ITIH4 Antibody - Anti-ITIH4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

IHCPlus™ ITIH4 Antibody aa744‑758 Polyclonal IHC,Peptide‑ELISA LS‑B5903

IHCPlus™ ITIH4 Antibody aa744‑758 Polyclonal IHC,Peptide‑ELISA LS‑B5903

Note: This antibody replaces LS-C112708
Antibody:
Goat Polyclonal to Human ITIH4
Application:
IHC, IHC-P, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B5903-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human ITIH4
Application:
IHC, IHC-P, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ITIH4 antibody LS-B5903 is an unconjugated goat polyclonal antibody to human ITIH4. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Target
Human ITIH4
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence ERLCVDPRHRQGPVN, from the internal region of the protein sequence according to NP_002209.2.
Blocking Peptide
LS-E26939 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5903. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa744-758
Specificity
Human ITIH4.
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments gave an approx. 150kDa band in Human Liver and Lung lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 103kDa according to NP_002209.2. The 150kDa band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ITIH4
Q14624 NM_002218 NP_002209.2

Validation

ITIH4 Antibody - Anti-ITIH4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-ITIH4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-ITIH4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

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Requested From: United States
Date Requested: 11/15/2019