Human IPAF / NLRC4

Rabbit

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity chromatography

Unmodified

Recombinant full length protein of human CARD12

Recognizes endogenous levels of CARD12 protein.

• IHC - Paraffin (1:50 - 1:200)
• ICC (1:50 - 1:200)
• Immunofluorescence (1:50 - 1:200)
• Western blot (1:500 - 1:2000)

Western blot analysis of CARD12 expression in MCF7 (A), mouse heart (B), rat kidney (C) whole cell lysates. Immunohistochemical analysis of CARD12 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Immunofluorescent analysis of CARD12 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9NPP4 NCBI: NM_021209 NP_067032.3