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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-B2951-50 50 µl (1 mg/ml) $425 
HSF1 Antibody - Anti-HSF1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
HSF1 Antibody - Cell lysates of HeLa(30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human HSF1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
HSF1 Antibody - Anti-HSF1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
HSF1 Antibody - Cell lysates of HeLa(30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human HSF1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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IHC‑plus™ Monoclonal Mouse anti‑Human HSF1 Antibody (clone J7F9, IHC, WB) LS‑B2951

IHC‑plus™ Monoclonal Mouse anti‑Human HSF1 Antibody (clone J7F9, IHC, WB) LS‑B2951

Note: This antibody replaces LS-C93795
Antibody:
HSF1 Mouse anti-Human Monoclonal (J7F9) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425
LS-B2951-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
HSF1 Mouse anti-Human Monoclonal (J7F9) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HSF1 antibody LS-B2951 is an unconjugated mouse monoclonal antibody to human HSF1. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HSF1
Synonyms
HSF1 | HSTF 1 | Heat shock factor protein 1 | HSF 1 | HSTF1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
J7F9
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
Recombinant human HSF1 (1-529 aa) purified from E. coli.
Applications
  • IHC
  • IHC - Paraffin (15 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2951 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2951 was determined to be 15 ug/ml.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSF1
Q00613 NM_005526 NP_005517.1

Validation

HSF1 Antibody - Anti-HSF1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
Anti-HSF1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
Anti-HSF1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.

LSBio Ratings

IHC-plus™ HSF1 Antibody (clone J7F9) for IHC, WB/Western, ELISA LS-B2951 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/26/2020