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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IHCPlus™ Histone H3.3 Antibody Monoclonal ELISA,IF,IHC,WB LS‑B8442

Catalog Number Size Price
LS-B8442-50 50 µg $495 
Anti-H3F3B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 15 ug/ml.
Immunofluorescence of monoclonal antibody to H3F3B on HeLa cell. [antibody concentration 10 ug/ml].
Western blot of H3F3B expression in transfected 293T cell line by H3F3B monoclonal antibody (M01), clone 2D7-H1.
Anti-H3F3B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 15 ug/ml.
Immunofluorescence of monoclonal antibody to H3F3B on HeLa cell. [antibody concentration 10 ug/ml].
Western blot of H3F3B expression in transfected 293T cell line by H3F3B monoclonal antibody (M01), clone 2D7-H1.
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IHCPlus™ Histone H3.3 Antibody Monoclonal ELISA,IF,IHC,WB LS‑B8442

Note: This antibody replaces LS-C133228
Antibody:
Mouse Monoclonal to Human Histone H3.3
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$495 
LS-B8442-50
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human Histone H3.3
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Histone H3.3 antibody LS-B8442 is an unconjugated mouse monoclonal antibody to human Histone H3.3. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Histone H3.3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
2D7-H1
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (15 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
H3F3B (AAH17558, 1 aa ~ 137 aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human H3F3B
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Validation

Anti-H3F3B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 15 ug/ml.
IHC Validated
Anti-H3F3B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 15 ug/ml.
Anti-H3F3B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 15 ug/ml.

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Requested From: United States
Date Requested: 8/24/2019
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