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Catalog Number Size Price
LS-B2891-50 50 µg (0.5 mg/ml) $440 
HEC1 / NDC80 Antibody - Anti-NDC80 / HEC1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
HEC1 / NDC80 Antibody - Anti-NDC80 / HEC1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
HEC1 / NDC80 Antibody - Antibody staining (0.5 ug/ml) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
HEC1 / NDC80 Antibody - Anti-NDC80 / HEC1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
HEC1 / NDC80 Antibody - Anti-NDC80 / HEC1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
HEC1 / NDC80 Antibody - Antibody staining (0.5 ug/ml) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
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IHC‑plus™ Polyclonal Goat anti‑Human HEC1 / NDC80 Antibody (aa630‑642, IHC, WB) LS‑B2891

IHC‑plus™ Polyclonal Goat anti‑Human HEC1 / NDC80 Antibody (aa630‑642, IHC, WB) LS‑B2891

Note: This antibody replaces LS-C54962, LS-C73203
Antibody:
HEC1 / NDC80 Goat anti-Human Polyclonal (aa630-642) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-B2891-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
HEC1 / NDC80 Goat anti-Human Polyclonal (aa630-642) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NDC80 antibody LS-B2891 is an unconjugated goat polyclonal antibody to human NDC80 (HEC1) (aa630-642). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HEC1 / NDC80
Synonyms
NDC80 | HEC1 | Kinetochore protein Hec1 | Kinetochore associated 2 | HEC | HsHec1 | HsNDC80 | KNTC2 | TID3
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-YEKKATLIKSSEE, from the C-Terminus of protein sequence according to NP_006092.1.
Epitope
aa630-642
Specificity
Human NDC80 / HEC1.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2891 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2891 was determined to be 5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HEC1 / NDC80
O14777 NM_006101 NP_006092.1

Validation

HEC1 / NDC80 Antibody - Anti-NDC80 / HEC1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NDC80 / HEC1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NDC80 / HEC1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

HEC1 / NDC80 Antibody - Anti-NDC80 / HEC1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NDC80 / HEC1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NDC80 / HEC1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ HEC1 / NDC80 Antibody (aa630-642) for IHC, WB/Western LS-B2891 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/16/2021