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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B1704-50 50 µl (3.44 mg/ml) $425 
HEC1 / NDC80 Antibody - Anti-NDC80 / HEC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC‑plus™ Monoclonal Mouse anti‑Human HEC1 / NDC80 Antibody (clone 9G3.23, aa56‑642, IHC, IF, WB) LS‑B1704

IHC‑plus™ Monoclonal Mouse anti‑Human HEC1 / NDC80 Antibody (clone 9G3.23, aa56‑642, IHC, IF, WB) LS‑B1704

Note: This antibody replaces LS-C20070
Antibody:
HEC1 / NDC80 Mouse anti-Human Monoclonal (aa56-642) (9G3.23) Antibody
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425
LS-B1704-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
HEC1 / NDC80 Mouse anti-Human Monoclonal (aa56-642) (9G3.23) Antibody
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NDC80 antibody LS-B1704 is an unconjugated mouse monoclonal antibody to human NDC80 (HEC1) (aa56-642). Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HEC1 / NDC80
Synonyms
NDC80 | HEC1 | Kinetochore protein Hec1 | Kinetochore associated 2 | HEC | HsHec1 | HsNDC80 | KNTC2 | TID3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Clone
9G3.23
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human NDC80 / HEC1.
Epitope
aa56-642
Specificity
Human HEC1 protein consisting of amino acids 56-642.
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence
  • Western blot (1:500 - 1:3000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B1704 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B1704 was determined to be 1:50.
Presentation
PBS
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HEC1 / NDC80
O14777 NM_006101 NP_006092.1

Validation

HEC1 / NDC80 Antibody - Anti-NDC80 / HEC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-NDC80 / HEC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-NDC80 / HEC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ HEC1 / NDC80 Antibody (aa56-642, clone 9G3.23) for IHC, IF/Immunofluorescence, WB/Western, IP LS-B1704 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 2/24/2021