Human H2AFX / H2AX
H2AFX | H2AX | Histone H2A.x | H2A.X | H2A histone family, member X | H2AX histone
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Synthetic peptide from human H2AFX / H2AX.
Amino acids surrounding Ser 139 of human H2AFX
- IHC - Paraffin (10 µg/ml)
- Western blot (1:500 - 1:1000)
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Immunohistochemistry: LS-B1474 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1474 was determined to be 10 ug/ml.
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.