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Catalog Number Size Price
LS-A5102-50 50 µg (1 mg/ml) $460 
GPR149 / PGR10 Antibody - Anti-GPR149 antibody IHC of human spinal cord, dorsal root ganglion. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC‑plus™ Polyclonal Rabbit anti‑Human GPR149 / PGR10 Antibody (Cytoplasmic Domain, IHC) LS‑A5102

IHC‑plus™ Polyclonal Rabbit anti‑Human GPR149 / PGR10 Antibody (Cytoplasmic Domain, IHC) LS‑A5102

Note: This antibody replaces LS-C66981
Antibody:
GPR149 / PGR10 Rabbit anti-Human Polyclonal (Cytoplasmic Domain) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$460
LS-A5102-50

Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
GPR149 / PGR10 Rabbit anti-Human Polyclonal (Cytoplasmic Domain) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
PGR10 antibody LS-A5102 is an unconjugated rabbit polyclonal antibody to human PGR10 (GPR149) (Cytoplasmic Domain). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human GPR149 / PGR10
Synonyms
GPR149 | PGR10 | G protein-coupled receptor 149 | IEDA
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human GPR149. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%); Elephant (94%); Monkey, Marmoset (88%); Bat (81%).
Epitope
Cytoplasmic Domain
Specificity
Human GPR149. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Applications
  • IHC
  • IHC - Paraffin (20 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 20 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GPR149 / PGR10
Q86SP6 NM_001038705 ABC49857.1

Validation

GPR149 / PGR10 Antibody - Anti-GPR149 antibody IHC of human spinal cord, dorsal root ganglion. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR149 antibody IHC of human spinal cord, dorsal root ganglion. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR149 antibody IHC of human spinal cord, dorsal root ganglion. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

IHC-plus™ GPR149 / PGR10 Antibody (Cytoplasmic Domain) for IHC, ELISA LS-A5102 has an LSBio Rating of
Laboratory Validation Score (4)

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Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/11/2022