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IHCPlus™ GPCRW / GPR18 Antibody Cytoplasmic Domain Polyclonal Biotin IHC LS‑A109459

Catalog Number Size Price
LS-A109459-100 100 µl $650 
Anti-GPR18 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHCPlus™ GPCRW / GPR18 Antibody Cytoplasmic Domain Polyclonal Biotin IHC LS‑A109459

Antibody:
Rabbit Polyclonal to Human GPCRW / GPR18
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Bat, Horse, Rabbit
Format:
Biotin, Unmodified
Other formats:
Price
Catalog Number
$650 
LS-A109459-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human GPCRW / GPR18
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Bat, Horse, Rabbit
Format:
Biotin, Unmodified
Other formats:

Specifications

Description
GPR18 antibody LS-A109459 is a biotin-conjugated rabbit polyclonal antibody to GPR18 (GPCRW ) from human, bat, horse and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human GPCRW / GPR18
Host
Rabbit
Reactivity
Human, Monkey, Bat, Horse, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin. Also available Unconjugated or conjugated with AP, APC, FITC, HRP, PE.  (This antibody is custom conjugated upon order. Following conjugation, the final antibody concentration will be determined and is typically > 0.5 mg/ml.)
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human GPR18. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat, Elephant, Horse, Rabbit, Pufferfish (100%); Mouse, Rat, Bovine, Pig, Opossum, Salmon, Zebrafish, Stickleback (94%); Chicken, Platypus, Lizard, Catfish (88%); Hamster, Turkey (81%).
Blocking Peptide
LS-E29672 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A109459. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Cytoplasmic Domain
Specificity
Human GPR18. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except ESYT3 (50%).
Usage
Immunohistochemistry: LS-A95 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A95 was determined to be 10 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GPCRW / GPR18
Q14330 NM_005292 NP_005283.1

Validation

Anti-GPR18 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-GPR18 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR18 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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Requested From: United States
Date Requested: 9/22/2019