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Catalog Number Size Price
LS-B15710-50 50 µl (1 mg/ml) $460 
GATM / AGAT Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
GATM / AGAT Antibody - Immunohistochemical analysis of AGAT staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
GATM / AGAT Antibody - Immunofluorescent analysis of AGAT staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
GATM / AGAT Antibody - Western blot analysis of AGAT expression in mouse kidney (A); rat kidney (B) whole cell lysates.
GATM / AGAT Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
GATM / AGAT Antibody - Immunohistochemical analysis of AGAT staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
GATM / AGAT Antibody - Immunofluorescent analysis of AGAT staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
GATM / AGAT Antibody - Western blot analysis of AGAT expression in mouse kidney (A); rat kidney (B) whole cell lysates.
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IHC‑plus™ Polyclonal Rabbit anti‑Human GATM / AGAT Antibody (IHC, IF, WB) LS‑B15710

IHC‑plus™ Polyclonal Rabbit anti‑Human GATM / AGAT Antibody (IHC, IF, WB) LS‑B15710

Note: This antibody replaces LS-C482652
Antibody:
GATM / AGAT Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B15710-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
GATM / AGAT Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
AGAT antibody LS-B15710 is an unconjugated rabbit polyclonal antibody to AGAT (GATM) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human GATM / AGAT
Synonyms
GATM | AGAT | AT | Glycine amidinotransferase | Transamidinase
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Recombinant full length human AGAT.
Specificity
Recognizes endogenous levels of AGAT protein.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
0.42% Potassium Phosphate, pH 7.3, 0.87% NaCl, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GATM / AGAT
P50440 NM_001482 NP_001473.1

Validation

GATM / AGAT Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ GATM / AGAT Antibody for IHC, ICC, IF/Immunofluorescence, WB/Western LS-B15710 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024