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Catalog Number Size Price
LS-B210-50 50 µg (1 mg/ml) $485 
FANCF Antibody - Anti-FANCF antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
FANCF Antibody - Anti-FANCF Antibody - Western Blot. Western blot of affinity purified anti-FANCF antibody shows detection of FANCF present in a lysate prepared from a Fanconi anemia complementation group F patient lymphoblast after retroviral correction using hFANCF cDNA (lanes 3 and 4). This band (indicated by arrowhead) is approximately 42.3 kD in size. The band is not detected in FA-F a lymphoblast lysate that is not corrected for the deletion and does not express the FANCF protein (lanes 1 and 2). Lanes 2 and 4 represent lysates taken from lymphoblasts after 40 J/m2 UV irradiation, whereas lanes 1 and 3 received no irradiation. No apparent difference was noted upon irradiation. The strong band at ~60kD appears to be non-specific. Personal communication, N. Howlett, University of Rhode Island, Kingston, RI.
FANCF Antibody - Anti-FANCF antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
FANCF Antibody - Anti-FANCF Antibody - Western Blot. Western blot of affinity purified anti-FANCF antibody shows detection of FANCF present in a lysate prepared from a Fanconi anemia complementation group F patient lymphoblast after retroviral correction using hFANCF cDNA (lanes 3 and 4). This band (indicated by arrowhead) is approximately 42.3 kD in size. The band is not detected in FA-F a lymphoblast lysate that is not corrected for the deletion and does not express the FANCF protein (lanes 1 and 2). Lanes 2 and 4 represent lysates taken from lymphoblasts after 40 J/m2 UV irradiation, whereas lanes 1 and 3 received no irradiation. No apparent difference was noted upon irradiation. The strong band at ~60kD appears to be non-specific. Personal communication, N. Howlett, University of Rhode Island, Kingston, RI.
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IHC‑plus™ Polyclonal Rabbit anti‑Human FANCF Antibody (Internal, IHC, WB) LS‑B210

IHC‑plus™ Polyclonal Rabbit anti‑Human FANCF Antibody (Internal, IHC, WB) LS‑B210

Note: This antibody replaces LS-C19024
Antibody:
FANCF Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Chimpanzee
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B210-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
FANCF Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Chimpanzee
Format:
Unconjugated, Unmodified

Specifications

Description
FANCF antibody LS-B210 is an unconjugated rabbit polyclonal antibody to FANCF (Internal) from human. It is reactive with human and chimpanzee. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human FANCF
Synonyms
FANCF | Fanconi anemia group F protein | Protein FACF | FAF
Host
Rabbit
Reactivity
Human, Chimpanzee (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal amino acid sequence of human FANCF.
Epitope
Internal
Specificity
A BLAST analysis was used to suggest cross-reactivity with FANCF protein from human and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:1000)
  • ELISA (1:15000 - 1:60000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B210 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B210 was determined to be 2.5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FANCF
Q9NPI8 NM_022725 NP_073562.1

Validation

FANCF Antibody - Anti-FANCF antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-FANCF antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-FANCF antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ FANCF Antibody (Internal) for IHC, WB/Western, ELISA LS-B210 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Development of a 3D functional assay and identification of biomarkers, predictive for response of high-grade serous ovarian cancer (HGSOC) patients to poly-ADP ribose polymerase inhibitors (PARPis): targeted therapy. Razan Sheta, Magdalena Bachvarova, Marie Plante, Marie-Claude Renaud, Alexandra Sebastianelli, Jean Gregoire, Jamilet Miranda Navarro, Ricardo Bringas Perez, Jean-Yves Masson, Dimcho Bachvarov. Journal of translational medicine. 2020 November;18:439.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022