FANCF | Fanconi anemia group F protein | Protein FACF | FAF
(tested or 100% immunogen sequence identity)
Chimpanzee (at least 90% immunogen sequence identity)
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal amino acid sequence of human FANCF.
A BLAST analysis was used to suggest cross-reactivity with FANCF protein from human and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
- IHC - Paraffin (2.5 µg/ml)
- Western blot (1:1000)
- ELISA (1:15000 - 1:60000)
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Immunohistochemistry: LS-B210 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B210 was determined to be 2.5 ug/ml.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
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