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IHCPlus™ ETFB Antibody aa231‑243 Polyclonal IHC,Peptide‑ELISA,WB LS‑B9220

Catalog Number Size Price
LS-B9220-50 50 µg (0.5 mg/ml) $425 
Anti-ETFB antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
ETFB antibody (0.01 ug/ml) staining of Mouse Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-ETFB antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
ETFB antibody (0.01 ug/ml) staining of Mouse Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHCPlus™ ETFB Antibody aa231‑243 Polyclonal IHC,Peptide‑ELISA,WB LS‑B9220

Note: This antibody replaces LS-C139564
Antibody:
Goat Polyclonal to Human ETFB
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Bat, Bovine, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B9220-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human ETFB
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Bat, Bovine, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
ETFB antibody LS-B9220 is an unconjugated goat polyclonal antibody to ETFB from human, mouse, bat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ETFB
Host
Goat
Reactivity
Human, Mouse, Bat, Bovine, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.01 - 0.03 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-PQRTAGVKVETTE, from the C Terminus of the protein sequence according to NP_001976.1NP_001014763.1.
Blocking Peptide
LS-E27411 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B9220. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa231-243
Specificity
Human ETFB. This antibody is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1). Amino acid numbering in the name above refers to NP_001976.1).
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 26kD band observed in Human, Mouse and Rat Liver lysates (calculated MW of 27.8kD according to NP_001976.1). Recommended concentration: 0.01-0.03 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ETFB
P38117 NM_001985 NP_001976.1

Validation

Anti-ETFB antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-ETFB antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-ETFB antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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Requested From: United States
Date Requested: 9/16/2019