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Catalog Number Size Price
LS-B11293-50 50 µl (1 mg/ml) $460 
EGLN3 / PHD3 Antibody - Anti-PHD3 / EGLN3 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
EGLN3 / PHD3 Antibody - Detection of human and mouse PHD3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHD3 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
EGLN3 / PHD3 Antibody - Detection of Human and Rat PHD3 by Western Blot. Samples: A. Lysate from cells that were transiently transfected with PHD1, PHD2, PHD3 or PHD4 constructs or in vitro translated PHD1, PHD2, PHD3, PHD4. B. Lysate from rat cells before or 9 hours after inducing hypoxia. Antibody: Affinity purified rabbit anti-PHD3 use at 1:1000 (A) or 1:5000 (B). Detection: Chemiluminescence.
EGLN3 / PHD3 Antibody - Anti-PHD3 / EGLN3 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
EGLN3 / PHD3 Antibody - Detection of human and mouse PHD3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHD3 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
EGLN3 / PHD3 Antibody - Detection of Human and Rat PHD3 by Western Blot. Samples: A. Lysate from cells that were transiently transfected with PHD1, PHD2, PHD3 or PHD4 constructs or in vitro translated PHD1, PHD2, PHD3, PHD4. B. Lysate from rat cells before or 9 hours after inducing hypoxia. Antibody: Affinity purified rabbit anti-PHD3 use at 1:1000 (A) or 1:5000 (B). Detection: Chemiluminescence.
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IHC‑plus™ Polyclonal Rabbit anti‑Human EGLN3 / PHD3 Antibody (aa50‑100, IHC, WB) LS‑B11293

IHC‑plus™ Polyclonal Rabbit anti‑Human EGLN3 / PHD3 Antibody (aa50‑100, IHC, WB) LS‑B11293

Note: This antibody replaces LS-C287227
Antibody:
EGLN3 / PHD3 Rabbit anti-Human Polyclonal (aa50-100) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B11293-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
EGLN3 / PHD3 Rabbit anti-Human Polyclonal (aa50-100) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
PHD3 antibody LS-B11293 is an unconjugated rabbit polyclonal antibody to PHD3 (EGLN3) (aa50-100) from human. It is reactive with human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human EGLN3 / PHD3
Synonyms
EGLN3 | HPH-1 | HIF prolyl hydroxylase 3 | HIF-prolyl hydroxylase 3 | HIFPH3 | PHD3 | EGL nine (C.elegans) homolog 3 | Egl nine homolog 3 | HIF-PH3 | HIFP4H3
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 112399 corresponding to amino acids between 50 and 100 of UniprotID: Q9H6Z9
Epitope
aa50-100
Specificity
Region between residue 50 and 100 of human Prolyl Hydroxylase Domain-Containing Protein 3 using the numbering given in entry NP_071356.1 (GeneID 112399).
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EGLN3 / PHD3
Q9H6Z9 NM_022073 NP_071356.1

Validation

EGLN3 / PHD3 Antibody - Anti-PHD3 / EGLN3 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PHD3 / EGLN3 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PHD3 / EGLN3 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ EGLN3 / PHD3 Antibody (aa50-100) for IHC, WB/Western LS-B11293 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/29/2024