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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IHCPlus™ DENND1B Antibody aa144‑193 Polyclonal IHC,WB LS‑B5833

Catalog Number Size Price
LS-B5833-100 100 µl $485 
Anti-DENND1B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DENND1B antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-DENND1B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DENND1B antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ DENND1B Antibody aa144‑193 Polyclonal IHC,WB LS‑B5833

Note: This antibody replaces LS-C102467
Antibody:
Rabbit Polyclonal to Human DENND1B
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Bat, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B5833-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human DENND1B
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Bat, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
DENND1B antibody LS-B5833 is an unconjugated rabbit polyclonal antibody to DENND1B from human, mouse, bat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human DENND1B
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Bat, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa144-193 of human DENND1B (Q6P3S1, NP_659414). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Dog, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Stickleback, Zebrafish (100%); Bovine (92%); Rat, Panda, Lizard, Xenopus, Beetle (83%).
Blocking Peptide
LS-E19011 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5833. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa144-193
Specificity
Human DENND1B
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DENND1B
Q6P3S1 NM_001142795 NP_659414.2

Validation

Anti-DENND1B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-DENND1B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-DENND1B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Anti-DENND1B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Bovine, Dog, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
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Species: Human
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Applications: IHC, Western blot, Flow Cytometry, ELISA
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Species: Human
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Requested From: United States
Date Requested: 8/22/2019
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