Target
Human DAP-5 / EIF4G2
Synonyms
EIF4G2 | AAG1 | Aging-associated protein 1 | DAP-5 | DAP5 | Death-associated protein 5 | EIF-4G 2 | EIF-4-gamma 2 | EIF4G 2 | Translation repressor nat1 | p97
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Two synthetic peptides corresponding to amino acids 796-812 of human DAP-5 protein.
Specificity
In Jurkat, a 97 kD band is observed.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (1 - 2 µg/ml)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B2026 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2026 was determined to be 10 ug/ml.
Presentation
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee