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IHCPlus™ CLTA + CLTB Antibody Internal Polyclonal IHC,Peptide‑ELISA,WB LS‑B6480

Catalog Number Size Price
LS-B6480-50 50 µg (0.5 mg/ml) $425 
Anti-CLTA/CLTB antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
CTLA + CTLB antibody (0.1µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Anti-CLTA/CLTB antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
CTLA + CTLB antibody (0.1µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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IHCPlus™ CLTA + CLTB Antibody Internal Polyclonal IHC,Peptide‑ELISA,WB LS‑B6480

Note: This antibody replaces LS-C87349
Antibody:
Goat Polyclonal to Human CLTA + CLTB
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B6480-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human CLTA + CLTB
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Format:
Unconjugated, Unmodified

Specifications

Description
CLTA + CLTB antibody LS-B6480 is an unconjugated goat polyclonal antibody to CLTA + CLTB from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CLTA + CLTB
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 - 3.75 µg/ml)
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence CDFNPKSSKQAKD, from the internal region of the protein sequence according to NP_001824.1NP_009027.1NP_001070145.1NP_001825.1NP_009028.1.
Blocking Peptide
LS-E26851 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B6480. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Internal
Specificity
Human CLTA. This antibody is expected to recognize all reported isoforms of light chains a and b.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx. 35kD and 22-23kD bands observed in Human Brain (Frontal Cortex) lysates (calculated MW of 23.2kD according to NP_001825.1). The observed molecular weight of ~35kD corresponds to earlier findings in literature with different antibodies (Okamoto et al, Am J Physiol Cell Physiol. 2000 Sep;279(3): C833-51.; PMID: 10942733). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Validation

Anti-CLTA/CLTB antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
IHC Validated
Anti-CLTA/CLTB antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-CLTA/CLTB antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

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Requested From: United States
Date Requested: 9/18/2019