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Catalog Number Size Price
LS-B16725-50 50 µg $460 
CDH1 / E Cadherin Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
CDH1 / E Cadherin Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
CDH1 / E Cadherin Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
CDH1 / E Cadherin Antibody - Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
CDH1 / E Cadherin Antibody - Immunofluorescence staining of HepG2 cells diluted at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
CDH1 / E Cadherin Antibody - Western Blot Positive WB detected in: 293 whole cell lysate All Lanes: CDH1 antibody at 2.9µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 98, 91 KDa Observed band size: 125 KDa
CDH1 / E Cadherin Antibody - Immunoprecipitating CDH1 in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of CDH1 Antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CDH1 Antibody (6µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (20µg)
CDH1 / E Cadherin Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
CDH1 / E Cadherin Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
CDH1 / E Cadherin Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
CDH1 / E Cadherin Antibody - Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
CDH1 / E Cadherin Antibody - Immunofluorescence staining of HepG2 cells diluted at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
CDH1 / E Cadherin Antibody - Western Blot Positive WB detected in: 293 whole cell lysate All Lanes: CDH1 antibody at 2.9µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 98, 91 KDa Observed band size: 125 KDa
CDH1 / E Cadherin Antibody - Immunoprecipitating CDH1 in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of CDH1 Antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CDH1 Antibody (6µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (20µg)
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IHC‑plus™ Polyclonal Rabbit anti‑Human CDH1 / E Cadherin Antibody (IHC, IF, WB) LS‑B16725

IHC‑plus™ Polyclonal Rabbit anti‑Human CDH1 / E Cadherin Antibody (IHC, IF, WB) LS‑B16725

Note: This antibody replaces LS-C680259
Antibody:
CDH1 / E Cadherin Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$460
LS-B16725-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CDH1 / E Cadherin Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
E Cadherin antibody LS-B16725 is an unconjugated rabbit polyclonal antibody to human E Cadherin (CDH1). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CDH1 / E Cadherin
Synonyms
CDH1 | CD324 | CDHE | Cell-CAM 120/80 | E Cadherin | E-Cadherin | ECAD | Epithelial cadherin | Arc-1 | UVO | Cadherin-1 | CAM 120/80 | CD324 antigen | LCAM | Uvomorulin
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant Human Cadherin-1 protein (23-621AA)
Applications
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDH1 / E Cadherin
P12830 NM_004360 NP_004351.1

Validation

CDH1 / E Cadherin Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

CDH1 / E Cadherin Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

CDH1 / E Cadherin Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ CDH1 / E Cadherin Antibody for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B16725 has an LSBio Rating of
Laboratory Validation Score (4)

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Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024