Human CD45 / LCA

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

F10-89-4

Unconjugated

Purified IgG

Unmodified

Human T lymphocytes

Recognizes the human CD45 cell surface antigen, also known as the leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc. Clone Reacts with all forms of CD45 expressed by all hematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. This product is routinely tested in flow cytometry on human peripheral blood leucocytes

• IHC - Paraffin (10 µg/ml)
• IHC - Frozen (1:500 - 1:1000)
• Immunoprecipitation
• Flow Cytometry (1:10 - 1:50)

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Immunohistochemistry: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1 mM EDTA pH8.0 is recommended for this purpose. Histology Positive Control Tissue: Human tonsil.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P08575 NCBI: NM_002838 NP_002829.3