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Catalog Number Size Price
LS-B335-50 50 µl (70 mg/ml) $485 
CAMK4 / CaMK IV Antibody - Anti-CAMK4 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
CAMK4 / CaMK IV Antibody - Anti-CaM Kinase IV Antibody - Western Blot. Western blot of Anti-CaM Kinase IV antibody shows detection of a band ~52 kD corresponding to CaM Kinase IV (arrowhead) in various preparations: lane 1 - rat brain lysate, lane 2 - Jurkat cell lysate. Specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 3 and 4 respectively). Approximately 35 ug of each lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CaM Kinase IV was similarly detected on lysates from mouse brain (not shown). After blocking the membrane was probed with the primary antibody diluted to 1:1000 for 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.
CAMK4 / CaMK IV Antibody - Specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 1 and 2 respectively).
CAMK4 / CaMK IV Antibody - Western blot of anti-CaM Kinase IV antibody shows detection of a band ~52 kDa corresponding to CaM Kinase IV (arrowhead) in various preparations.  Staining of rat brain lysate is shown in lane 1.  Jurkat cell lysate staining is shown in lane 2.
CAMK4 / CaMK IV Antibody - Anti-CAMK4 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
CAMK4 / CaMK IV Antibody - Anti-CaM Kinase IV Antibody - Western Blot. Western blot of Anti-CaM Kinase IV antibody shows detection of a band ~52 kD corresponding to CaM Kinase IV (arrowhead) in various preparations: lane 1 - rat brain lysate, lane 2 - Jurkat cell lysate. Specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 3 and 4 respectively). Approximately 35 ug of each lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CaM Kinase IV was similarly detected on lysates from mouse brain (not shown). After blocking the membrane was probed with the primary antibody diluted to 1:1000 for 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.
CAMK4 / CaMK IV Antibody - Specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 1 and 2 respectively).
CAMK4 / CaMK IV Antibody - Western blot of anti-CaM Kinase IV antibody shows detection of a band ~52 kDa corresponding to CaM Kinase IV (arrowhead) in various preparations.  Staining of rat brain lysate is shown in lane 1.  Jurkat cell lysate staining is shown in lane 2.
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IHC‑plus™ Polyclonal Rabbit anti‑Human CAMK4 / CaMK IV Antibody (IHC, WB) LS‑B335

IHC‑plus™ Polyclonal Rabbit anti‑Human CAMK4 / CaMK IV Antibody (IHC, WB) LS‑B335

Note: This antibody replaces LS-C18758
Antibody:
CAMK4 / CaMK IV Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Chimpanzee, Mouse, Rat, Bovine, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B335-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
CAMK4 / CaMK IV Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Chimpanzee, Mouse, Rat, Bovine, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
CaMK IV antibody LS-B335 is an unconjugated rabbit polyclonal antibody to CaMK IV (CAMK4) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CAMK4 / CaMK IV
Synonyms
CAMK4 | CAM kinase IV | CaMK IV | CaMK-GR | CAMKIV | CaMK | CAM kinase- GR | CaM kinase-GR | CaM-KIV
Host
Rabbit
Reactivity
Human, Chimpanzee, Mouse, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
This antiserum was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 300-325 of Human CaM Kinase IV protein.
Specificity
This antiserum is directed against human CaM Kinase IV protein. The product was delipidated, defibrinated followed by buffering and clarification. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, and rat based on 100% homology for the immunogen sequence. Cross reactivity with CaM Kinase IV homologues from other sources has not been determined.
Applications
  • IHC
  • IHC - Paraffin (1:500)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:25000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B335 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B335 was determined to be 1:500.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CAMK4 / CaMK IV
Q16566 NM_001744 NP_001735.1

Validation

CAMK4 / CaMK IV Antibody - Anti-CAMK4 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-CAMK4 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-CAMK4 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

LSBio Ratings

IHC-plus™ CAMK4 / CaMK IV Antibody for IHC, WB/Western, ELISA LS-B335 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/28/2021