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IHCPlus™ CA1 / Carbonic Anhydrase I Antibody N‑Terminus Polyclonal IHC,WB LS‑B10420

Catalog Number Size Price
LS-B10420-100 100 µl $485 
Anti-CA1 / Carbonic Anhydrase I antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CA1 / Carbonic Anhydrase I antibody Western blot of Placenta lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CA1 / Carbonic Anhydrase I antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CA1 / Carbonic Anhydrase I antibody Western blot of Placenta lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ CA1 / Carbonic Anhydrase I Antibody N‑Terminus Polyclonal IHC,WB LS‑B10420

Note: This antibody replaces LS-C102777
Antibody:
Rabbit Polyclonal to Human CA1 / Carbonic Anhydrase I
Application:
IHC, IHC-P, WB
Reactivity:
Human, Bovine, Guinea pig
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B10420-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CA1 / Carbonic Anhydrase I
Application:
IHC, IHC-P, WB
Reactivity:
Human, Bovine, Guinea pig
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Carbonic Anhydrase I antibody LS-B10420 is an unconjugated rabbit polyclonal antibody to Carbonic Anhydrase I (CA1 ) from human, bovine and guinea pig. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CA1 / Carbonic Anhydrase I
Host
Rabbit
Reactivity
Human, Bovine, Guinea pig (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from N-Terminus of human CA1 (P00915, NP_001729). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%); Marmoset (92%); Elephant (91%); Galago (83%); Rat (78%).
Blocking Peptide
LS-E21368 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B10420. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human CA1 / Carbonic Anhydrase I
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CA1 / Carbonic Anhydrase I
P00915 NM_001738 NP_001729.1

Validation

Anti-CA1 / Carbonic Anhydrase I antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-CA1 / Carbonic Anhydrase I antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CA1 / Carbonic Anhydrase I antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Requested From: United States
Date Requested: 9/22/2019