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IHCPlus™ BRS3 Antibody C‑Terminus Polyclonal Azide‑free HRP IHC LS‑A102819

Catalog Number Size Price
LS-A102819-100 100 µl $650 
Anti-BRS3 antibody IHC of human pancreas, islet. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHCPlus™ BRS3 Antibody C‑Terminus Polyclonal Azide‑free HRP IHC LS‑A102819

Antibody:
Rabbit Polyclonal to Human BRS3
Application:
IHC, IHC-P
Reactivity:
Human
Format:
Horseradish Peroxidase, Azide-free
Other formats:
Price
Catalog Number
$650 
LS-A102819-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human BRS3
Application:
IHC, IHC-P
Reactivity:
Human
Format:
Horseradish Peroxidase, Azide-free
Other formats:

Specifications

Description
BRS3 antibody LS-A102819 is an HRP-conjugated rabbit polyclonal antibody to human BRS3. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human BRS3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with AP, APC, Biotin, FITC, PE.  (This antibody is custom conjugated upon order. Following conjugation, the final antibody concentration will be determined and is typically > 0.5 mg/ml.)
Purification
Immunoaffinity purified
Modifications
Azide-free
Applications
  • IHC
  • IHC - Paraffin
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 20 amino acid peptide from C-terminal cytoplasmic domain of human BRS3. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Gibbon (95%); Monkey (90%); Bat, Elephant (85%); Horse, Rabbit, Guinea pig (80%).
Blocking Peptide
LS-E28553 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A102819. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human BRS3. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GRPR (45%).
Usage
Immunohistochemistry: LS-A24 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A24 was determined to be 10 ug/ml.
Presentation
PBS
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BRS3
P32247 NM_001727 NP_001718.1

Validation

Anti-BRS3 antibody IHC of human pancreas, islet. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-BRS3 antibody IHC of human pancreas, islet. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-BRS3 antibody IHC of human pancreas, islet. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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Requested From: United States
Date Requested: 8/23/2019
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