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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B8532-50 50 µl (1 mg/ml) $425 
API5 Antibody - Anti-API5 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
API5 Antibody - Western blot analysis of lysates from 293, Jurkat, and HUVEC cells, using API-5 Antibody. The lane on the right is blocked with the synthesized peptide.
API5 Antibody - Anti-API5 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
API5 Antibody - Western blot analysis of lysates from 293, Jurkat, and HUVEC cells, using API-5 Antibody. The lane on the right is blocked with the synthesized peptide.
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IHCPlus™ Polyclonal Rabbit anti‑Human API5 Antibody (aa421‑470, IHC, WB) LS‑B8532

IHCPlus™ Polyclonal Rabbit anti‑Human API5 Antibody (aa421‑470, IHC, WB) LS‑B8532

Note: This antibody replaces LS-C119388
Antibody:
Rabbit Polyclonal to Human API5
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B8532-50
50 µl
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human API5
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
API5 antibody LS-B8532 is an unconjugated rabbit polyclonal antibody to API5 (aa421-470) from human. It is reactive with human and mouse. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human API5
Synonyms
API5 | AAC11 | AAC-11 | Antiapoptosis clone 11 protein | API-5 | Apoptosis inhibitor 5 | FIF | API5L1 | Protein XAGL
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from human API-5. (AA Range: 421-470)
Epitope
aa421-470
Specificity
API-5 Antibody detects endogenous levels of total API-5 protein.
Usage
Western Blot: Predicted MW 57 kDa
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About API5
Q9BZZ5 NM_006595 NP_006586.1

Validation

API5 Antibody - Anti-API5 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
Anti-API5 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
Anti-API5 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.

LSBio Ratings

IHC-plus™ API5 Antibody (aa421-470) for IHC, WB/Western LS-B8532 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 5/31/2020