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Catalog Number Size Price
LS-B9566-50 50 µg (0.5 mg/ml) $440 
AP2A1 / AP2-Alpha Antibody - Anti-AP2A1 / AP2-Alpha antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
AP2A1 / AP2-Alpha Antibody - AP2A1 antibody (0.1 ug/ml) staining of methanol-fixed HeLa cells with stably expressing Mouse Ap2a1. Primary incubation was 1 hour. Detected by Alexa Fluor 594. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK
AP2A1 / AP2-Alpha Antibody - AP2A1 antibody (0.1 ug/ml) staining of Mouse Brain lysate (~5 ug protein in SDSPAGE buffer). The left panel shows the same blot stained with Ponceau red for total protein stain as the loading control before labeling. Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK
AP2A1 / AP2-Alpha Antibody - AP2A1 antibody (0.01 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
AP2A1 / AP2-Alpha Antibody - Anti-AP2A1 / AP2-Alpha antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
AP2A1 / AP2-Alpha Antibody - AP2A1 antibody (0.1 ug/ml) staining of methanol-fixed HeLa cells with stably expressing Mouse Ap2a1. Primary incubation was 1 hour. Detected by Alexa Fluor 594. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK
AP2A1 / AP2-Alpha Antibody - AP2A1 antibody (0.1 ug/ml) staining of Mouse Brain lysate (~5 ug protein in SDSPAGE buffer). The left panel shows the same blot stained with Ponceau red for total protein stain as the loading control before labeling. Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK
AP2A1 / AP2-Alpha Antibody - AP2A1 antibody (0.01 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHC‑plus™ Polyclonal Goat anti‑Human AP2A1 / AP2‑Alpha Antibody (aa706‑727, IHC, WB) LS‑B9566

IHC‑plus™ Polyclonal Goat anti‑Human AP2A1 / AP2‑Alpha Antibody (aa706‑727, IHC, WB) LS‑B9566

Note: This antibody replaces LS-C186441
Antibody:
AP2A1 / AP2-Alpha Goat anti-Human Polyclonal (aa706-727) Antibody
Application:
IHC, IHC-P, ICC, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Dog, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-B9566-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
AP2A1 / AP2-Alpha Goat anti-Human Polyclonal (aa706-727) Antibody
Application:
IHC, IHC-P, ICC, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Dog, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
AP2-Alpha antibody LS-B9566 is an unconjugated goat polyclonal antibody to AP2-Alpha (AP2A1) (aa706-727) from human. It is reactive with human, mouse, dog and other species. Validated for ICC, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human AP2A1 / AP2-Alpha
Synonyms
AP2A1 | Adaptin, alpha A | ADTAA | Alpha-adaptin A | Alpha1-adaptin | AP-2 complex subunit alpha-1 | AP2 Alpha | CLAPA1 | AP2-alpha
Host
Goat
Reactivity
Human, Monkey, Mouse, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-ELEPPAPESPMALLADPAPAAD, from the internal region of the protein sequence according to NP_055018.2.
Epitope
aa706-727
Specificity
Human AP2A1 / AP2-Alpha. This antibody is expected to recognize reported isoform 1 (NP_055018.2) only.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot (0.01 - 0.03 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 110kD band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 108kD according to NP_055018.2). The same band is observed in Mouse Brain, but not in Mouse Liver or Mouse Kidney lysates. Recommended concentration: 0.01-0.03 ug/ml. Immunocytochemistry: HeLa stably expressing a chimeric construct including the brain-specific splice variant of Mouse Ap2a1, showing clathrin-coated pits and vesicles budding from the plasma membrane.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AP2A1 / AP2-Alpha
O95782 NM_014203 NP_055018.2

Validation

AP2A1 / AP2-Alpha Antibody - Anti-AP2A1 / AP2-Alpha antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AP2A1 / AP2-Alpha antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AP2A1 / AP2-Alpha antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ AP2A1 / AP2-Alpha Antibody (aa706-727) for IHC, ICC, WB/Western LS-B9566 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/4/2022