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Catalog Number Size Price
LS-B327-50 50 µg (1 mg/ml) $485 
ANAPC10 / APC10 Antibody - Anti-ANAPC10 / APC10 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ANAPC10 / APC10 Antibody - Anti-APC10 Antibody - Western Blot. Affinity Purified Rabbit anti-APC10 was used at a 1:500 dilution to detect human APC10 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. Approximately 20 ug of each lysate was loaded onto a 10% SDS-PAGE gel. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 5 min. The expected molecular weight of human APC10 is 21 kD.
ANAPC10 / APC10 Antibody - Anti-APC10 Antibody - Western Blot. Affinity Purified Rabbit anti-APC10 was used at a 1:500 dilution to detect human APC10 in various cell extracts. This antibody clearly detects a ~26 kD band corresponding to human APC10 (predicted molecular weight is 21 kD). All lanes contain 20 ug of lysate or extract as follows: lane 1, HeLa nuclear extract; lane 2, HeLa whole cell lysate; lane 3, A431 whole cell lysate; lane 4, Jurkat whole cell lysate; lane 5, 293 whole cell lysate. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 4 min.
ANAPC10 / APC10 Antibody - Anti-ANAPC10 / APC10 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ANAPC10 / APC10 Antibody - Anti-APC10 Antibody - Western Blot. Affinity Purified Rabbit anti-APC10 was used at a 1:500 dilution to detect human APC10 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. Approximately 20 ug of each lysate was loaded onto a 10% SDS-PAGE gel. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 5 min. The expected molecular weight of human APC10 is 21 kD.
ANAPC10 / APC10 Antibody - Anti-APC10 Antibody - Western Blot. Affinity Purified Rabbit anti-APC10 was used at a 1:500 dilution to detect human APC10 in various cell extracts. This antibody clearly detects a ~26 kD band corresponding to human APC10 (predicted molecular weight is 21 kD). All lanes contain 20 ug of lysate or extract as follows: lane 1, HeLa nuclear extract; lane 2, HeLa whole cell lysate; lane 3, A431 whole cell lysate; lane 4, Jurkat whole cell lysate; lane 5, 293 whole cell lysate. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 4 min.
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IHC‑plus™ Polyclonal Rabbit anti‑Human ANAPC10 / APC10 Antibody (IHC, WB) LS‑B327

IHC‑plus™ Polyclonal Rabbit anti‑Human ANAPC10 / APC10 Antibody (IHC, WB) LS‑B327

Note: This antibody replaces LS-C19103, LS-C69101
Antibody:
ANAPC10 / APC10 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B327-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
ANAPC10 / APC10 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
APC10 antibody LS-B327 is an unconjugated rabbit polyclonal antibody to human APC10 (ANAPC10). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ANAPC10 / APC10
Synonyms
ANAPC10 | Cyclosome subunit 10 | DKFZP564L0562 | APC10 | DOC1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of human APC10.
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:500 - 1:1500)
  • Immunoprecipitation (1:100)
  • ELISA (1:15000 - 1:80000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B327 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B327 was determined to be 2.5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ANAPC10 / APC10
Q9UM13 NM_014885 NP_055700.2

Validation

ANAPC10 / APC10 Antibody - Anti-ANAPC10 / APC10 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ANAPC10 / APC10 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ANAPC10 / APC10 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ ANAPC10 / APC10 Antibody for IHC, WB/Western, IP, ELISA LS-B327 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/27/2021