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Catalog Number Size Price
LS-B326-50 50 µl (10 mg/ml) $440 
ALPI / Alkaline Phosphatase Antibody - Anti-ALPI / Alkaline Phosphatase antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ALPI / Alkaline Phosphatase Antibody - Anti-Alkaline Phosphatase Antibody - Western Blot. Both the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the 60000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (LS-C60884) diluted 1:4000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.
ALPI / Alkaline Phosphatase Antibody - Anti-ALPI / Alkaline Phosphatase antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ALPI / Alkaline Phosphatase Antibody - Anti-Alkaline Phosphatase Antibody - Western Blot. Both the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the 60000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (LS-C60884) diluted 1:4000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.
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IHC‑plus™ Polyclonal Rabbit anti‑Human ALPI / Alkaline Phosphatase Antibody (IHC, WB) LS‑B326

IHC‑plus™ Polyclonal Rabbit anti‑Human ALPI / Alkaline Phosphatase Antibody (IHC, WB) LS‑B326

Note: This antibody replaces LS-C19070
Antibody:
ALPI / Alkaline Phosphatase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-B326-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
ALPI / Alkaline Phosphatase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Alkaline Phosphatase antibody LS-B326 is an unconjugated rabbit polyclonal antibody to human Alkaline Phosphatase (ALPI). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ALPI / Alkaline Phosphatase
Synonyms
ALPI | Alkaline phosphomonoesterase | Kasahara isozyme | Glycerophosphatase | Alkaline Phosphatase | IAP
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified IgG
Modifications
Unmodified
Immunogen
Purified alkaline phosphatase from human intestine
Specificity
Alkaline Phosphatase [Human Intestine].
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B326 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B326 was determined to be 2.5 ug/ml.
Presentation
0.01 % sodium azide, 0.02 M potassium phosphate, 0.15 M sodium chloride, dH2O.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ALPI / Alkaline Phosphatase
P09923 NM_001631 NP_001622.2

Validation

ALPI / Alkaline Phosphatase Antibody - Anti-ALPI / Alkaline Phosphatase antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ALPI / Alkaline Phosphatase antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ALPI / Alkaline Phosphatase antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ ALPI / Alkaline Phosphatase Antibody for IHC, WB/Western, ELISA LS-B326 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/17/2021