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IHCPlus™ ADGRE5 / CD97 Antibody N‑Terminus Polyclonal AP ICC,IHC LS‑A103001

Catalog Number Size Price
LS-A103001-100 100 µl $650 
Anti-CD97 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human Thyroid, Papillary Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human bone marrow. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody immunocytochemistry (ICC) staining of HEK293 human embryonic kidney cells transfected with CD97.
Anti-CD97 antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.
Anti-CD97 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human Thyroid, Papillary Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human bone marrow. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody immunocytochemistry (ICC) staining of HEK293 human embryonic kidney cells transfected with CD97.
Anti-CD97 antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.
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IHCPlus™ ADGRE5 / CD97 Antibody N‑Terminus Polyclonal AP ICC,IHC LS‑A103001

Antibody:
Rabbit Polyclonal to Human ADGRE5 / CD97
Application:
IHC, IHC-P, ICC
Reactivity:
Human
Format:
Alkaline Phosphatase, Unmodified
Other formats:
Price
Catalog Number
$650 
LS-A103001-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human ADGRE5 / CD97
Application:
IHC, IHC-P, ICC
Reactivity:
Human
Format:
Alkaline Phosphatase, Unmodified
Other formats:

Specifications

Description
CD97 antibody LS-A103001 is an AP-conjugated rabbit polyclonal antibody to human CD97 (ADGRE5 ). Validated for ICC and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human ADGRE5 / CD97
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Alkaline Phosphatase. Also available Unconjugated or conjugated with APC, Biotin, FITC, HRP, PE.  (This antibody is custom conjugated upon order. Following conjugation, the final antibody concentration will be determined and is typically > 0.5 mg/ml.)
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 19 amino acid peptide from N-terminal extracellular domain of human CD97. Percent identity with other species by BLAST analysis: Human (100%); Monkey (95%).
Blocking Peptide
LS-E28584 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A103001. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human CD97. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MAP3K12 (58%).
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADGRE5 / CD97
P48960 NM_078481 NP_510966.1

Validation

Anti-CD97 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-CD97 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-CD97 antibody IHC of human Thyroid, Papillary Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-CD97 antibody IHC of human Thyroid, Papillary Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human Thyroid, Papillary Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-CD97 antibody IHC of human bone marrow. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-CD97 antibody IHC of human bone marrow. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human bone marrow. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-CD97 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-CD97 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD97 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-CD97 antibody immunocytochemistry (ICC) staining of HEK293 human embryonic kidney cells transfected with CD97.
IHC Validated
Anti-CD97 antibody immunocytochemistry (ICC) staining of HEK293 human embryonic kidney cells transfected with CD97.
Anti-CD97 antibody immunocytochemistry (ICC) staining of HEK293 human embryonic kidney cells transfected with CD97.

Anti-CD97 antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.
IHC Validated
Anti-CD97 antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.
Anti-CD97 antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.

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Featured Products

Anti-CD97 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot
Anti-CD97 Antibody - Western Blot. Western blot of Protein A purified anti-CD97 antibody shows detection of bands corresponding to free Fc-CD97- (5EGF) (lower arrowhead) and Fc-CD97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells. The left lane contains lysate from cells transfected with control DNA. The right lane contains lysate from COS cells expressing Fc-CD97- (5EGF). No staining was noted from bone marrow lysates taken from CD97 knockout mice. The identity of the band at ~65 kD appearing in all lanes is not known. The formation of the CD97 complex is currently under investigation. Approximately 10 ul of lysate was used in each lane. A 1:1000 dilution of the primary antibody was used. The image was processed using a 10-sec exposure. Personal Communication. Yvona Ward. NIH, NCI, CCR, Bethesda, MD.
Species: Mouse, Human
Applications: Immunofluorescence, Western blot
Reactivity: Mouse
Range: 0.27-200 pg/ml
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-WWTR1 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Reactivity: Human
Range: 7.8-500 ng/ml
Reactivity: Pig
Range: 1.56-100 ng/ml

Requested From: United States
Date Requested: 8/21/2019
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