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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IGHM / IgM Antibody LS-C680644

IgM antibody LS-C680644 is an unconjugated rabbit polyclonal antibody to human IgM (IGHM). Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C680644-50
50 µg
$285
LS-C680644-100
100 µg
$385
Description
IgM antibody LS-C680644 is an unconjugated rabbit polyclonal antibody to human IgM (IGHM). Validated for ELISA, IHC and WB.
Target
Human IGHM / IgM
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
IGHM / IgM antibody was raised against recombinant Human Immunoglobulin heavy constant mu protein (45-178AA)
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only.
About IGHM / IgM
P01871 P01871

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Images

Immunohistochemistry - Paraffin

Immunohistochemistry Dilution at 1:600 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:600 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Western blot

Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, MCF-7 whole cell lysate, U87 whole cell lysate All Lanes: IGHM antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 52 KDa Observed band size: 50 KDa
Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, MCF-7 whole cell lysate, U87 whole cell lysate All Lanes: IGHM antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 52 KDa Observed band size: 50 KDa

Immunohistochemistry - Paraffin

Immunohistochemistry Dilution at 1:600 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:600 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Western blot

Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, MCF-7 whole cell lysate, U87 whole cell lysate All Lanes: IGHM antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 52 KDa Observed band size: 50 KDa
Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, MCF-7 whole cell lysate, U87 whole cell lysate All Lanes: IGHM antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 52 KDa Observed band size: 50 KDa

Immunohistochemistry - Paraffin

Immunohistochemistry Dilution at 1:600 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:600 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Western blot

Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, MCF-7 whole cell lysate, U87 whole cell lysate All Lanes: IGHM antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 52 KDa Observed band size: 50 KDa
Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, MCF-7 whole cell lysate, U87 whole cell lysate All Lanes: IGHM antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 52 KDa Observed band size: 50 KDa

Immunohistochemistry - Paraffin

Immunohistochemistry Dilution at 1:600 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:600 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Western blot

Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, MCF-7 whole cell lysate, U87 whole cell lysate All Lanes: IGHM antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 52 KDa Observed band size: 50 KDa
Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, MCF-7 whole cell lysate, U87 whole cell lysate All Lanes: IGHM antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 52 KDa Observed band size: 50 KDa

Requested From: United States
Date Requested: 4/26/2019
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