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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C346706-1 1 ml $475 
Antibody

Polyclonal Goat anti‑Rat IgG1 + IgG2a + IgG2b + IgG2c Fc Antibody (HRP, IHC) LS‑C346706

Polyclonal Goat anti‑Rat IgG1 + IgG2a + IgG2b + IgG2c Fc Antibody (HRP, IHC) LS‑C346706

Antibody:
IgG1 + IgG2a + IgG2b + IgG2c Fc Goat anti-Rat Polyclonal (HRP) Antibody
Application:
IHC, IHC-P, ICC, ELISA, DB, ID
Reactivity:
Rat
Format:
Horseradish Peroxidase, Unmodified
Other formats:
Price
Catalog Number
$475
LS-C346706-1
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
IgG1 + IgG2a + IgG2b + IgG2c Fc Goat anti-Rat Polyclonal (HRP) Antibody
Application:
IHC, IHC-P, ICC, ELISA, DB, ID
Reactivity:
Rat
Format:
Horseradish Peroxidase, Unmodified
Other formats:

Specifications

Description
IgG1 + IgG2a + IgG2b + IgG2c Fc antibody LS-C346706 is an HRP-conjugated goat polyclonal antibody to rat IgG1 + IgG2a + IgG2b + IgG2c Fc. Validated for DB, ELISA, ICC, ID and IHC.
Target
Rat IgG1 + IgG2a + IgG2b + IgG2c Fc
Host
Goat
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with FITC, Biotin, TRITC.
Purification
Purified IgG
Modifications
Unmodified
Immunogen
Purified normal IgG, including all subclasses, isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Rat IgG, Fc specific.
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • ELISA
  • Dot Blot
  • Immunodiffusion
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum contains antibodies to subclass-specific determinants as well as to determinants shared by two or more subclasses of IgG. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal rat plasma, serum, and immunoglobulin fractions, no reaction is obtained with IgA, IgM and gG/Fab fragments or any other serum protein. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen using a reference antibody of rat origin known to be of the IgG isotype in the middle layer of the indirect test procedure. in non-isotopic assay methodology (e. g. ELISA) to measure IgG in rat serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500. in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:10000.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/26/2020