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Catalog Number Size Price
LS-C346709-1 1 ml $372 
Antibody

Polyclonal Goat anti‑Rat IgG1 + IgG2a + IgG2b + IgG2c Antibody (Biotin, IHC) LS‑C346709

Polyclonal Goat anti‑Rat IgG1 + IgG2a + IgG2b + IgG2c Antibody (Biotin, IHC) LS‑C346709

Antibody:
IgG1 + IgG2a + IgG2b + IgG2c Goat anti-Rat Polyclonal (Biotin) Antibody
Application:
IHC, IHC-P, ICC, ELISA, DB, ID
Reactivity:
Rat
Format:
Biotin, Unmodified
Other formats:
Price
Catalog Number
$372
LS-C346709-1
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
IgG1 + IgG2a + IgG2b + IgG2c Goat anti-Rat Polyclonal (Biotin) Antibody
Application:
IHC, IHC-P, ICC, ELISA, DB, ID
Reactivity:
Rat
Format:
Biotin, Unmodified
Other formats:

Specifications

Description
IgG1 + IgG2a + IgG2b + IgG2c antibody LS-C346709 is a biotin-conjugated goat polyclonal antibody to rat IgG1 + IgG2a + IgG2b + IgG2c. Validated for DB, ELISA, ICC, ID and IHC.
Target
Rat IgG1 + IgG2a + IgG2b + IgG2c
Host
Goat
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin. Also available Unconjugated or conjugated with HRP, TRITC, FITC.
Purification
Purified IgG
Modifications
Unmodified
Immunogen
Purified normal IgG, including all known subclasses, isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Rat IgG, heavy and light chains.
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • ELISA
  • Dot Blot
  • Immunodiffusion
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500. in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:10000.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/23/2022