Rat IgE Fc

Goat

Rat (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with Biotin, HRP, TRITC, FITC.

Purified IgG

Unmodified

Purified homogenous IgE isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Goat Rat IgE, Fc specific.

• Immunofluorescence
• ELISA
• Dot Blot

The reactivity of the antiserum is directed to the Fc subunits of the IgE molecule which represents strict isotype (class) specificity. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against appropriate concentrations of IgE, a single precipitin line is obtained which shows a reaction of full identity with the precipitin line obtained with the used immunogen. It does not react with rat IgG, including all subclasses, IgA, IgM and IgG/Fab or any non-Ig protein in rat serum. As unlabelled secondary antibody for indirect detection of IgE in rat cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods. for the production of immunoconjugates with a selected marker. to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier. as catching or detecting antibody reagent in non-isotopic assay methodology (e. g. ELISA) to identify and measure IgE in rat serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5000.

Lyophilized from PBS, pH 7.2, No preservatives added

Reconstitute with distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.