Pig IgA, Secretory

Rabbit

Pig (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Antiserum

Unmodified

Highly purified secretory IgA isolated from pooled swine milk. Freund's complete adjuvant is used in the first step of the immunization procedure.

Swine secretory IgA.

• Immunodiffusion

The reactivity of the antiserum is restricted to the Fc part of the IgA molecule and the surface and hidden determinants of the secretory component. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal swine milk two precipitin lines may be obtained, one representing IgA and the other the free secretory component. Against normal swine serum only IgA is precipitated. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in swine milk or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of swine IgA from serum or exocrine secretions. Antisera to IgA or sIgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude the use of the antiserum in more sensitive techniques if proper controls are included.

Delipidated, heat-inactivated, lyophilized antiserum. No preservative.

1 ml Distilled water

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.