Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C344975-100 100 µg $424 
IDO1 / IDO Antibody - Immunocytochemistry fluorescent staining of human mature dendritic cells using anti-IDO (human), pAb .
IDO1 / IDO Antibody - Western blot analysis of human IDO using anti-IDO (human), pAb at 1:2,000 dilution.1. Recombinant human IDO (His tagged).2. PHA- treated human peripheral blood lymphocyte lysate.
IDO1 / IDO Antibody - LPS induction of IDO in THP-1 cells: To examine the ability of induction of IDO with LPS, reactions were carried out at 37 degrees C over 8, 16, 20, 24, 32, 40, 48hrs, respectively by adding LPS (1 ug/ml) to THP-1 cells, which were not maintained with serum starvation. LPS in lanes 2, 3, 4, 5, 6, 7, and 8 were subjected to LPS-responsive THP-1 cell treatments over 8, 16, 20, 24, 32, 40, 48hrs, respectively. Lane 1 was not treated with LPS. IDO was detected by using anti-IDO (human), pAb .
IDO1 / IDO Antibody - Immunocytochemistry fluorescent staining of human mature dendritic cells using anti-IDO (human), pAb .
IDO1 / IDO Antibody - Western blot analysis of human IDO using anti-IDO (human), pAb at 1:2,000 dilution.1. Recombinant human IDO (His tagged).2. PHA- treated human peripheral blood lymphocyte lysate.
IDO1 / IDO Antibody - LPS induction of IDO in THP-1 cells: To examine the ability of induction of IDO with LPS, reactions were carried out at 37 degrees C over 8, 16, 20, 24, 32, 40, 48hrs, respectively by adding LPS (1 ug/ml) to THP-1 cells, which were not maintained with serum starvation. LPS in lanes 2, 3, 4, 5, 6, 7, and 8 were subjected to LPS-responsive THP-1 cell treatments over 8, 16, 20, 24, 32, 40, 48hrs, respectively. Lane 1 was not treated with LPS. IDO was detected by using anti-IDO (human), pAb .
1 of 3
2 of 3
3 of 3

Polyclonal Rabbit anti‑Human IDO1 / IDO Antibody (IF, WB) LS‑C344975

Polyclonal Rabbit anti‑Human IDO1 / IDO Antibody (IF, WB) LS‑C344975

Antibody:
IDO1 / IDO Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB, Flo, ELISA, Func
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$424
LS-C344975-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IDO1 / IDO Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB, Flo, ELISA, Func
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IDO antibody LS-C344975 is an unconjugated rabbit polyclonal antibody to human IDO (IDO1). Validated for ELISA, Flow, Func, ICC, IF and WB.
Target
Human IDO1 / IDO
Synonyms
IDO1 | IDO | Indole 2,3-dioxygenase | Indolamine 2,3 dioxygenase | IDO-1 | INDO | Indoleamine 2,3-dioxygenase | Indoleamine 2,3-dioxygenase 1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Recombinant human IDO.
Specificity
Recognizes human IDO. Detects a band of ~45kDa by Western blot.
Applications
  • ICC (1:500)
  • Immunofluorescence
  • Western blot (1:2000 - 1:5000)
  • Flow Cytometry (1:200 - 1:500)
  • ELISA (1:2000 - 1:10000)
  • Functional Assay
Usage
ELISA - Direct and indirect: 1:2000-1:10000. Flow Cytometry - 1:200-1:500. Immunocytochemistry - 1:500. Western Blot - 1:2000-1:5000 using ECL. suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. suggested incubation time is 1 hour at room temperature.
Presentation
PBS, pH 7.4, no preservatives.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IDO1 / IDO
P14902 NM_002164 AAA36081.1

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024