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Catalog Number Size Price
LS-C745260-25 25 µl (0.95 mg/ml) $275 
IDO1 / IDO Antibody - Immunohistochemistry of Mouse Anti-IDO1 Antibody. Tissue: epididymis from wild-type (left) or IDO1 null mice (right). Fixation: frozen sections. Antigen retrieval: not required. Primary antibody: IDO1 (2E2) monoclonal antibody. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: IDO-1 is located in the cytosol. Staining: IDO-1 as precipitated brown signal.
IDO1 / IDO Antibody - Immunohistochemistry of Mouse anti-IDO1 antibody. Tissue: epididymis from wild-type (left) or IDO1 null mice (right). Fixation: paraffin-embedded. Primary antibody: IDO1 (2E2) monoclonal antibody. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: IDO-1 is located in the cytosol. Staining: IDO1 as precipitated brown signal.
IDO1 / IDO Antibody - Immunofluorescence Microscopy of Mouse anti-IDO1 Antibody. Cells: HEK293 cells. Fixation: 0.5% PFA. Expressing: mouse IDO-1 (left) and mouse IDO-2 (right). Primary antibody: IDO1 (2E2) monoclonal antibody. Antigen retrieval: not required. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: IDO-1 is located in the cytosol. Staining: IDO1 as red fluorescent signal with bis-benzimide nuclear counterstain (blue).
IDO1 / IDO Antibody - Western Blot of mouse anti-IDO1 antibody. Lane 1: HEK293 control vector. Lane 2: HEK293 expressing mouse IDO1. Lane 3: HEK293 expressing mouse IDO2. Load: 35 µg per lane. Primary antibody: IDO 1 antibody at 1:400 for overnight at 4°C. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 45.6 kDa, ~44 kDa for IDO1. Other band(s): unspecifics.
IDO1 / IDO Antibody - Western Blot of Mouse Anti-IDO1 antibody. Extracts from 293HEK Cells expressing: Lane 1: Control Vector. Lane 2: His-tagged mouse IDO1. Lane 3: mouse IDO1. Lane 4: His-tagged mouse IDO2. Lane 5: mouse IDO2. Lane 6: Epididymis from IDO null. Lane 7: wild type mice. Primary antibody: IDO-1(2E2) monoclonal antibody. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 1xPBST overnight at 4°C. Predicted/Observed size: 41-42 kDa/41-42 kDa for IDO-1. Other band(s): none.
IDO1 / IDO Antibody - Flow Cytometry of Mouse anti-IDO1 antibody. Cells: HEK293 cells. Expresing: mouse IDO-1(blue) and mouse IDO-2 (red). Primary antibody: IDO1 (2E2) monoclonal antibody. Secondary antibody: Biotin mouse secondary antibody at 1:10,000 for 45 min at RT and streptavidin PE at 1:5,000 for 30 min at RT.
IDO1 / IDO Antibody - Immunohistochemistry of Mouse Anti-IDO1 Antibody. Tissue: epididymis from wild-type (left) or IDO1 null mice (right). Fixation: frozen sections. Antigen retrieval: not required. Primary antibody: IDO1 (2E2) monoclonal antibody. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: IDO-1 is located in the cytosol. Staining: IDO-1 as precipitated brown signal.
IDO1 / IDO Antibody - Immunohistochemistry of Mouse anti-IDO1 antibody. Tissue: epididymis from wild-type (left) or IDO1 null mice (right). Fixation: paraffin-embedded. Primary antibody: IDO1 (2E2) monoclonal antibody. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: IDO-1 is located in the cytosol. Staining: IDO1 as precipitated brown signal.
IDO1 / IDO Antibody - Immunofluorescence Microscopy of Mouse anti-IDO1 Antibody. Cells: HEK293 cells. Fixation: 0.5% PFA. Expressing: mouse IDO-1 (left) and mouse IDO-2 (right). Primary antibody: IDO1 (2E2) monoclonal antibody. Antigen retrieval: not required. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: IDO-1 is located in the cytosol. Staining: IDO1 as red fluorescent signal with bis-benzimide nuclear counterstain (blue).
IDO1 / IDO Antibody - Western Blot of mouse anti-IDO1 antibody. Lane 1: HEK293 control vector. Lane 2: HEK293 expressing mouse IDO1. Lane 3: HEK293 expressing mouse IDO2. Load: 35 µg per lane. Primary antibody: IDO 1 antibody at 1:400 for overnight at 4°C. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 45.6 kDa, ~44 kDa for IDO1. Other band(s): unspecifics.
IDO1 / IDO Antibody - Western Blot of Mouse Anti-IDO1 antibody. Extracts from 293HEK Cells expressing: Lane 1: Control Vector. Lane 2: His-tagged mouse IDO1. Lane 3: mouse IDO1. Lane 4: His-tagged mouse IDO2. Lane 5: mouse IDO2. Lane 6: Epididymis from IDO null. Lane 7: wild type mice. Primary antibody: IDO-1(2E2) monoclonal antibody. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 1xPBST overnight at 4°C. Predicted/Observed size: 41-42 kDa/41-42 kDa for IDO-1. Other band(s): none.
IDO1 / IDO Antibody - Flow Cytometry of Mouse anti-IDO1 antibody. Cells: HEK293 cells. Expresing: mouse IDO-1(blue) and mouse IDO-2 (red). Primary antibody: IDO1 (2E2) monoclonal antibody. Secondary antibody: Biotin mouse secondary antibody at 1:10,000 for 45 min at RT and streptavidin PE at 1:5,000 for 30 min at RT.
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Monoclonal Mouse anti‑Mouse IDO1 / IDO Antibody (clone 2E2.6, IHC, IF, WB) LS‑C745260

Monoclonal Mouse anti‑Mouse IDO1 / IDO Antibody (clone 2E2.6, IHC, IF, WB) LS‑C745260

Antibody:
IDO1 / IDO Mouse anti-Mouse Monoclonal (2E2.6) Antibody
Application:
IHC, IF, WB, IP, Flo, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745260-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
IDO1 / IDO Mouse anti-Mouse Monoclonal (2E2.6) Antibody
Application:
IHC, IF, WB, IP, Flo, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
IDO antibody LS-C745260 is an unconjugated mouse monoclonal antibody to mouse IDO (IDO1). Validated for ELISA, Flow, IF, IHC, IP and WB.
Target
Mouse IDO1 / IDO
Synonyms
IDO1 | IDO | Indole 2,3-dioxygenase | Indolamine 2,3 dioxygenase | IDO-1 | INDO | Indoleamine 2,3-dioxygenase | Indoleamine 2,3-dioxygenase 1
Host
Mouse
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Non-Reactivity
Human
Clonality
IgG1 Monoclonal
Clone
2E2.6
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Unmodified
Immunogen
IDO1 antibody was produced in mouse by repeated immunizations with mouse recombinant IDO1 protein followed by hybridoma development.
Applications
  • IHC
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:500 - 1:1500)
  • Immunoprecipitation
  • Flow Cytometry
  • ELISA (1:5000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IDO1 / IDO
P14902 NM_002164 AAA36081.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 5/6/2021