Products
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
206-374-1102
Fax:
206-577-4565
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, Proforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C45179-25 25 µg (1 mg/ml) $363 
LS-C45179-0.2 0.2 mg (1 mg/ml) $504 
Antibody

Monoclonal Mouse anti‑Human ICAM‑1 / CD54 Antibody (clone 84H10, IHC) LS‑C45179

Monoclonal Mouse anti‑Human ICAM‑1 / CD54 Antibody (clone 84H10, IHC) LS‑C45179

Note: This antibody replaces LS-C58525
Antibody:
ICAM-1 / CD54 Mouse anti-Human Monoclonal (84H10) Antibody
Application:
IHC, IHC-Fr, IP, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$363
LS-C45179-25
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ICAM-1 / CD54 Mouse anti-Human Monoclonal (84H10) Antibody
Application:
IHC, IHC-Fr, IP, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD54 antibody LS-C45179 is an unconjugated mouse monoclonal antibody to human CD54 (ICAM-1). Validated for ELISA, Flow, IHC and IP.
Target
Human ICAM-1 / CD54
Synonyms
ICAM1 | BB2 | CD54 antigen | CD54 | ICAM-1 | p3.58
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
84H10
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
K562 cell line.
Specificity
Recognizes the D1 domain of ICAM-1. Reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1 beta or IFN gamma on other cell types such as fibroblasts and endothelial cells. Detects an antigen of 90kD. Clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1. Removal of sodium azide is recommended prior to use in functional assays - XXX recommend the use of EQU003 for this purpose.
Applications
  • IHC
  • IHC - Frozen (1:20 - 1:50)
  • Immunoprecipitation
  • Flow Cytometry (1:1 - 1:10)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.
Presentation
PBS, 0.09% Sodium Azide
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ICAM-1 / CD54
P05362 NM_000201 NP_000192.2

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022