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Catalog Number Size Price
LS-C45357-0.5 0.5 mg (1 mg/ml) $645 
ICAM-1 / CD54 Antibody - Staining of frozen rat spleen with Mouse anti-Rat CD54 (MOUSE ANTI RAT CD54).  This image was taken for the unmodified form of this product. Other forms have not been tested.

Monoclonal Mouse anti‑Rat ICAM‑1 / CD54 Antibody (clone 1A29, Low Endotoxin, IHC, IF, WB) LS‑C45357

Monoclonal Mouse anti‑Rat ICAM‑1 / CD54 Antibody (clone 1A29, Low Endotoxin, IHC, IF, WB) LS‑C45357

Antibody:
ICAM-1 / CD54 Mouse anti-Rat Monoclonal (Low Endotoxin) (1A29) Antibody
Application:
IHC-P, IHC-Fr, IF, WB, IP, Flo
Reactivity:
Rat
Format:
Unconjugated, Low Endotoxin
Other formats:
Price
Catalog Number
$645
LS-C45357-0.5
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ICAM-1 / CD54 Mouse anti-Rat Monoclonal (Low Endotoxin) (1A29) Antibody
Application:
IHC-P, IHC-Fr, IF, WB, IP, Flo
Reactivity:
Rat
Format:
Unconjugated, Low Endotoxin
Other formats:

Specifications

Description
CD54 antibody LS-C45357 is an unconjugated mouse monoclonal antibody to rat CD54 (ICAM-1). Validated for Flow, IF, IHC, IP and WB.
Target
Rat ICAM-1 / CD54
Synonyms
ICAM1 | BB2 | CD54 antigen | CD54 | ICAM-1 | p3.58
Host
Mouse
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
1A29
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Low Endotoxin. Also available Unmodified.
Immunogen
Rat Ax cells (a HEV derived cell line).
Specificity
Recognizes the rat CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1), a 90kD adhesion molecule belonging to the immunoglobulin superfamily. CD54 is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system. Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling. Functionally 1A29 inhibits homotypic aggregation of PHA blasts.
Applications
  • IHC - Paraffin
  • IHC - Frozen
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry (1:25 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Immunohistology: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Presentation
PBS
Storage
Store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ICAM-1 / CD54
P05362 NM_000201 NP_000192.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022