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| Catalog Number | Size | Price |
|---|---|---|
| LS-C186519-100 | 100 µg (0.5 mg/ml) | $503 |
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Polyclonal Goat anti‑Human ICAM‑1 / CD54 Antibody (aa94‑105, IF, WB) LS‑C186519
Polyclonal Goat anti‑Human ICAM‑1 / CD54 Antibody (aa94‑105, IF, WB) LS‑C186519
Antibody:
ICAM-1 / CD54 Goat anti-Human Polyclonal (aa94-105) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
ICAM-1 / CD54 Goat anti-Human Polyclonal (aa94-105) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
CD54 antibody LS-C186519 is an unconjugated goat polyclonal antibody to human CD54 (ICAM-1) (aa94-105). Validated for IF, Peptide-ELISA and WB.
Target
Human ICAM-1 / CD54
Synonyms
ICAM1 | BB2 | CD54 antigen | CD54 | ICAM-1 | p3.58
Host
Goat
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence SNCPDGQSTAKT, from the internal region of the protein sequence according to NP_000192.2.
Epitope
aa94-105
Specificity
Human ICAM1 / ICAM-1 / CD54. The immunizing peptide represents part of the extracellular domain, and it does not overlap any known glycosite.
Applications
- Immunofluorescence (5 µg/ml)
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:1000)
Usage
Peptide ELISA: antibody detection limit dilution 1:1000. Western blot: Approx 100kD band observed in lysates of cell lines Daudi and HeLa (calculated MW of 57.8kD according to NP_000192.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pino et al, Reproduction. 2009 Nov;138(5):837-47. PMID: 19661147). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ICAM-1 / CD54
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Rat, Bovine
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Species:
Human
Applications:
Western blot, Flow Cytometry, ELISA
Species:
Human, Baboon, Monkey, Cynomolgus
Applications:
IHC, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Species:
Human
Applications:
Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Species:
Rat
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
Date Requested: 4/27/2024
