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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C15667-50 50 µl $435 
LS-C15667-200 200 µl $755 
Antibody

Polyclonal Rabbit anti‑Human HSPB1 / HSP27 Antibody (phospho‑Ser82, WB) LS‑C15667

Polyclonal Rabbit anti‑Human HSPB1 / HSP27 Antibody (phospho‑Ser82, WB) LS‑C15667

Antibody:
Rabbit Polyclonal to Human HSPB1 / HSP27
Application:
WB, IP
Reactivity:
Human, Bovine, Dog, Guinea pig, Sheep, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$435 
LS-C15667-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human HSPB1 / HSP27
Application:
WB, IP
Reactivity:
Human, Bovine, Dog, Guinea pig, Sheep, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
HSP27 antibody LS-C15667 is an unconjugated rabbit polyclonal antibody to HSP27 (HSPB1) (pSer82) from human. It is reactive with human, bovine, chicken and other species. Validated for IP and WB.
Target
Human HSPB1 / HSP27
Synonyms
HSPB1 | 28 kDa heat shock protein | Heat shock 27 kDa protein | Heat shock 27kD protein 1 | Heat shock 27kDa protein 1 | HSP27 | HSP28 | HSP 27 | Hsp25 | SRP27 | Stress-responsive protein 27 | CMT2F | Heat shock 27 kd protein | Heat shock protein beta-1 | HMN2B | HS.76067
Host
Rabbit
Reactivity
Human, Bovine, Dog, Guinea pig, Sheep, Chicken (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation (1:50)
Immunogen
An 11 residue synthetic peptide, LSRQL[pS]SGVSE(C) corresponding to amino acids 77-87 of human Hsp27 with a cysteine (C) residue added and the peptide coupled to KLH.
Epitope
pSer82
Specificity
Detects an ~27kD protein, corresponding to the apparent molecular mass of phosphorylated Hsp27 (Ser82) on SDS-PAGE immunoblots. Species cross-reactivity: human, bovine, canine, chicken, guinea pig and sheep. This antibody is specific for phosphorylated Hsp27 (Ser82) and does not exhibit reactivity with non-phosphorylated Hsp27. Specificity for phospho-Hsp27 was demonstrated in peptide competition studies with phosphorylated and non-phosphorylated peptides.
Usage
Suitable for use in Western Blot and Immunoprecipitation. Western Blot (Colorimetric): 1:500. Immunoprecipitation: 1:50.
Presentation
Supplied as a liquid.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPB1 / HSP27
P04792 NM_001540 NP_001531.1

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Requested From: United States
Date Requested: 7/11/2020