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Catalog Number Size Price
LS-C762230-10 10 µl $295 
LS-C762230-100 100 µl (1 mg/ml) $514 
HSPB1 / HSP27 Antibody - Detection of human HSP27 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HSP27 used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB
HSPB1 / HSP27 Antibody - Detection of human HSP27 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HSP27 antibody used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
HSPB1 / HSP27 Antibody - Detection of human HSP27 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HSP27 used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB
HSPB1 / HSP27 Antibody - Detection of human HSP27 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HSP27 antibody used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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Polyclonal Rabbit anti‑Human HSPB1 / HSP27 Antibody (aa150‑200, IHC, WB) LS‑C762230

Polyclonal Rabbit anti‑Human HSPB1 / HSP27 Antibody (aa150‑200, IHC, WB) LS‑C762230

Antibody:
HSPB1 / HSP27 Rabbit anti-Human Polyclonal (aa150-200) Antibody
Application:
IHC, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295
LS-C762230-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HSPB1 / HSP27 Rabbit anti-Human Polyclonal (aa150-200) Antibody
Application:
IHC, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HSP27 antibody LS-C762230 is an unconjugated rabbit polyclonal antibody to human HSP27 (HSPB1) (aa150-200). Validated for IHC, IP and WB.
Target
Human HSPB1 / HSP27
Synonyms
HSPB1 | 28 kDa heat shock protein | Heat shock 27 kDa protein | Heat shock 27kD protein 1 | Heat shock 27kDa protein 1 | HSP27 | HSP28 | HSP 27 | Hsp25 | SRP27 | Stress-responsive protein 27 | CMT2F | Heat shock 27 kd protein | Heat shock protein beta-1 | HMN2B | HS.76067
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 3315 corresponding to ammino acids between 150 and 200 of UniprotID: P04792
Epitope
aa150-200
Applications
  • IHC (1:2000 - 1:10000)
  • Western blot (1:1000 - 1:5000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunoprecipitation Range: 2-10 µg/mg lysate. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2°C to 8°C for up to 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPB1 / HSP27
P04792 NM_001540 NP_001531.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 5/7/2024