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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HSPA6 / HSP70B' Antibody LS‑C482863
HSP70B' antibody LS-C482863 is an unconjugated rabbit polyclonal antibody to HSP70B' (HSPA6) from human, mouse and rat. Validated for IHC and WB.
Catalog
Size
Price
LS-C482863-50
50 µl (1 mg/ml)
$255
LS-C482863-100
100 µl (1 mg/ml)
$295
LS-C482863-200
200 µl (1 mg/ml)
$375

Popular HSPA6 / HSP70B' Products

Antibody
Species: Human
Applications: Western blot
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Anti-HSPA6 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA6.
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Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

HSP70B' antibody LS-C482863 is an unconjugated rabbit polyclonal antibody to HSP70B' (HSPA6) from human, mouse and rat. Validated for IHC and WB.

Specifications

Target
Human HSPA6 / HSP70B'
Synonyms
HSPA6, Heat shock 70 kd protein b, Heat shock 70 kDa protein 6, Heat shock 70 kDa protein B, Heat shock 70 kd protein 7, HSP70B
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HSPA6 / HSP70B' antibody was raised against recombinant full length human HSPA6.
Specificity
Recognizes endogenous levels of HSPA6 protein.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About HSPA6 / HSP70B'
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. P17066 NM_002155 NP_002146.2


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Images

Immunohistochemistry

Immunohistochemical analysis of HSPA6 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of HSPA6 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot

Western blot analysis of HSPA6 expression in Jurkat (A); mouse heart (B); rat brain (C) whole cell lysates.
Western blot analysis of HSPA6 expression in Jurkat (A); mouse heart (B); rat brain (C) whole cell lysates.

Immunohistochemistry

Immunohistochemical analysis of HSPA6 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of HSPA6 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot

Western blot analysis of HSPA6 expression in Jurkat (A); mouse heart (B); rat brain (C) whole cell lysates.
Western blot analysis of HSPA6 expression in Jurkat (A); mouse heart (B); rat brain (C) whole cell lysates.

Immunohistochemistry

Immunohistochemical analysis of HSPA6 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of HSPA6 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot

Western blot analysis of HSPA6 expression in Jurkat (A); mouse heart (B); rat brain (C) whole cell lysates.
Western blot analysis of HSPA6 expression in Jurkat (A); mouse heart (B); rat brain (C) whole cell lysates.

Immunohistochemistry

Immunohistochemical analysis of HSPA6 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of HSPA6 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot

Western blot analysis of HSPA6 expression in Jurkat (A); mouse heart (B); rat brain (C) whole cell lysates.
Western blot analysis of HSPA6 expression in Jurkat (A); mouse heart (B); rat brain (C) whole cell lysates.

Requested From: United States
Date Requested: 11/12/2018

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