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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HSPA6 / HSP70B' Antibody (aa377‑628) IHC‑plus™ LS‑B10826
Note: This antibody replaces LS-C185768
HSP70B' antibody LS-B10826 is an unconjugated rabbit polyclonal antibody to human HSP70B' (HSPA6). Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B10826-50
50 µl (1 mg/ml)
$425

Popular HSPA6 / HSP70B' Products

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Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

HSP70B' antibody LS-B10826 is an unconjugated rabbit polyclonal antibody to human HSP70B' (HSPA6). Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human HSPA6 / HSP70B'
Synonyms
HSPA6, Heat shock 70 kd protein b, Heat shock 70 kDa protein 6, Heat shock 70 kDa protein B, Heat shock 70 kd protein 7, HSP70B
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (7.5 µg/ml)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HSPA6 / HSP70B' antibody was raised against recombinant fragment corresponding to a region within amino acids 377 and 628 of HSPA6 (SwissProt P17066).
Epitope
aa377-628
Specificity
Human HSPA6
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
About HSPA6 / HSP70B'
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. P17066 NM_002155 NP_002146.2


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Images

Immunohistochemistry

Anti-HSPA6 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
Anti-HSPA6 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using HSPA6 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using HSPA6 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: H1299. 7.5% SDS PAGE. HSPA6 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: H1299. 7.5% SDS PAGE. HSPA6 antibody diluted at 1:1000

Immunohistochemistry

Anti-HSPA6 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
Anti-HSPA6 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using HSPA6 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using HSPA6 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: H1299. 7.5% SDS PAGE. HSPA6 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: H1299. 7.5% SDS PAGE. HSPA6 antibody diluted at 1:1000

Immunohistochemistry

Anti-HSPA6 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
Anti-HSPA6 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using HSPA6 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using HSPA6 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: H1299. 7.5% SDS PAGE. HSPA6 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: H1299. 7.5% SDS PAGE. HSPA6 antibody diluted at 1:1000

Immunohistochemistry

Anti-HSPA6 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
Anti-HSPA6 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using HSPA6 antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using HSPA6 antibody at 1:200 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: H1299. 7.5% SDS PAGE. HSPA6 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: H1299. 7.5% SDS PAGE. HSPA6 antibody diluted at 1:1000

Requested From: United States
Date Requested: 11/12/2018

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