Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
HSD11B2, -HSD11 type II, 11-beta-HSD, 11-beta-HSD2, 11-DH2, AME1, AME, HSD2, SDR9C3, HSD11K
(tested or 100% immunogen sequence identity)
Western blot (1 - 10 µg/ml)
ELISA (1:10000 - 1:100000)
HSD2 / HSD11B2 antibody was raised against a 19-aa synthetic peptide mapping near the C-terminus of rat 11b-HSD2 (1), coupled to KLH.
Recognizes Hydroxysteroid Dehydrogenase, 11-beta Type II. No significant sequence homology is seen with 11b-HSD1 or other proteins. Species cross-reactivity: The rat peptide is not well conserved in mouse and human 11b-HSD2. We recommend the use for mouse and human 11b-HSD2, LS-C24605 and LS-C24599 respectively. See LS-C24604 for anti-HSD Type I.
Suitable for use in ELISA and Western Blot. Western Blot: 1-10 ug/ml (ECL). ELISA: 1:10000-1:100000 using 50-100 ng of control peptide/well.
There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity.