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Antibodies > HSD17B10 / HADH2 > LS-C757607
 
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Catalog Number Size Price
LS-C757607-200 200 µl $393 
HSD17B10 / HADH2 Antibody - HSD17B10 Antibody (Center) staining HSD17B10 in human thyroid carcinoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
HSD17B10 / HADH2 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling HSD17B10 with HSD17B10 Antibody (Center) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (OI17558410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
HSD17B10 / HADH2 Antibody - All lanes: Anti-HSD17B10 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: K562 whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: SH-SY5Y whole cell lysate Lane 6: rat brain lysate Lane 7: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
HSD17B10 / HADH2 Antibody - Overlay histogram showing Hela cells stained with HSD17B10 Antibody (Center) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (HSD17B10 Antibody (Center), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
HSD17B10 / HADH2 Antibody - HSD17B10 Antibody (Center) staining HSD17B10 in human thyroid carcinoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
HSD17B10 / HADH2 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling HSD17B10 with HSD17B10 Antibody (Center) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (OI17558410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
HSD17B10 / HADH2 Antibody - All lanes: Anti-HSD17B10 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: K562 whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: SH-SY5Y whole cell lysate Lane 6: rat brain lysate Lane 7: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
HSD17B10 / HADH2 Antibody - Overlay histogram showing Hela cells stained with HSD17B10 Antibody (Center) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (HSD17B10 Antibody (Center), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Polyclonal Rabbit anti‑Human HSD17B10 / HADH2 Antibody (Internal, IHC, IF, WB) LS‑C757607

Polyclonal Rabbit anti‑Human HSD17B10 / HADH2 Antibody (Internal, IHC, IF, WB) LS‑C757607

Submit Review LSBio Guarantee
Antibody:
HSD17B10 / HADH2 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC-P, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C757607-200
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
HSD17B10 / HADH2 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC-P, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HADH2 antibody LS-C757607 is an unconjugated rabbit polyclonal antibody to HADH2 (HSD17B10) (Internal) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IF, IHC and WB.
Target
Human HSD17B10 / HADH2
Synonyms
HSD17B10 | 17-beta-HSD 10 | 17b-HSD10 | CAMR | DUPXp11.22 | ABAD | ERAB | HADH2 | MHBD | MRPP2 | MRX17 | SCHAD | SDR5C1 | MRX31 | MRXS10 | Type II HADH | HCD2 | XH98G2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Bovine (at least 90% immunogen sequence identity)
Clonality
Ig Polyclonal
Conjugations
Unconjugated
Purification
Protein A and Peptide Affinity Chromatography
Modifications
Unmodified
Immunogen
This HSD17B10 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 140-172 amino acids from the Central region of human HSD17B10.
Epitope
Internal
Applications
  • IHC - Paraffin (1:25)
  • Immunofluorescence (1:25)
  • Western blot (1:2000)
  • Flow Cytometry (1:25)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Western Blot Predicted Molecular Weight: 26.9 kDa
Presentation
PBS, 0.09% Sodium Azide
Storage
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSD17B10 / HADH2
Q99714 NM_004493 NP_004484.1

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Featured Products

HSD17B10 / HADH2 Antibody - IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HSD17B10 mouse monoclonal antibody.
HSD17B10 / HADH2 Antibody (clone OTI11A2) LS‑C114806
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
HSD17B10 / HADH2 Antibody - Anti-HSD17B10 / HADH2 antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC‑plus™ HSD17B10 / HADH2 Antibody (aa242‑252) LS‑B9190
Species: Human, Mouse, Rat, Bovine, Hamster, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
HSD17B10 / HADH2 Antibody - Immunohistochemical analysis of HADH2 staining in human lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
HSD17B10 / HADH2 Antibody (Internal) LS‑C352301
Species: Human, Mouse, Rat, Bovine, Dog, Pig
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
HSD17B10 / HADH2 Antibody - Western blot. All lanes: 3-hydroxyacyl-CoA dehydrogenase type-2 antibody at 2 ug/ml Lane 1:mouse brain tissue Lane 2: 293T whole cell lysate. Lane 3: HepG2 whole cell lysate Lane 4: HeLa whole cell lysate Lane 5: HT29 whole cell lysate. Secondary antibody: Goat polyclonal. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HSD17B10 / HADH2 Antibody (aa2‑261, HRP) LS‑C371996
Species: Human
Applications: Western blot, ELISA
HSD17B10 / HADH2 ELISA Kit
Human HSD17B10 / HADH2 (Sandwich ELISA) ELISA Kit - LS-F11610
Reactivity: Human
Range: 0.156-10 ng/ml
HSD17B10 / HADH2 Protein
Human HSD17B10 / HADH2 Protein (Recombinant His) - LS-G740
Source: E. coli
Tag: His
See all HSD17B10 / HADH2 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/29/2024
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