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Catalog Number Size Price
LS-C153963-2 2 ml (45 mg/ml) $417 
HRP / Horseradish Peroxidase Antibody - Anti-Peroxidase Polyclonal Antibody-Western blot. Goat anti-Peroxidase antibody was used to detect horse radish peroxidase under reducing and non-reducing conditions. Reduced samples of purified horse radish peroxidase contained 4% BME and were boiled for 5 minutes. Samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Goat anti-Peroxidase antibody (1:5K in MB-0070, overnight at 4C). Primary antibody was detected with Dylight 649 conjugated Donkey anti-Goat (1:10K 1.5 hr RT in MB-070 and imaged on the Bio-Rad VersaDoc imaging system.

Polyclonal Goat anti‑Horseradish HRP / Horseradish Peroxidase Antibody (WB) LS‑C153963

Polyclonal Goat anti‑Horseradish HRP / Horseradish Peroxidase Antibody (WB) LS‑C153963

Antibody:
HRP / Horseradish Peroxidase Goat anti-Horseradish Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Horseradish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$417
LS-C153963-2
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HRP / Horseradish Peroxidase Goat anti-Horseradish Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Horseradish
Format:
Unconjugated, Unmodified

Specifications

Description
Horseradish Peroxidase antibody LS-C153963 is an unconjugated goat polyclonal antibody to horseradish Horseradish Peroxidase (HRP). Validated for ELISA and WB.
Target
HRP / Horseradish Peroxidase
Synonyms
HRP
Host
Goat
Reactivity
Horseradish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
Peroxidase [Horseradish].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Peroxidase [Horseradish]. Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.
Applications
  • Western blot (1:900 - 1:4000)
  • ELISA (1:9000 - 1:40000)
Usage
This product has been assayed against 1.0 ug of Peroxidase [Horseradish] in a standard sandwich ELISA using Alkaline Phosphatase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and pNPP as a substrate for 30 minutes at room temperature. A working dilution of 1:9000 to 1:40000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 ug of Peroxidase [Horseradish] in a standard sandwich ELISA using Alkaline Phosphatase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and pNPP as a substrate for 30 minutes at room temperature. A working dilution of 1:9000 to 1:40000 of the reconstitution concentration is suggested for this product.
Presentation
Lyophilized from 0.01 M Sodium Phosphate, pH 7.2, 0.15 M NaCl
Reconstitution
Reconstitute in 2 mL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/29/2024