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Catalog Number Size Price
LS-C662083-10 10 µg $318 
LS-C662083-100 100 µg $470 
HPSE / Heparanase Antibody - Western blot analysis of Heparanase 1 using anti-Heparanase 1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Heparanase 1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Heparanase 1 at approximately 65KD. The expected band size for Heparanase 1 is at 61KD.
HPSE / Heparanase Antibody - Western blot - Anti-Heparanase 1 Picoband Antibody
HPSE / Heparanase Antibody - Western blot analysis of Heparanase 1 using anti-Heparanase 1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Heparanase 1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Heparanase 1 at approximately 65KD. The expected band size for Heparanase 1 is at 61KD.
HPSE / Heparanase Antibody - Western blot - Anti-Heparanase 1 Picoband Antibody
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Polyclonal Rabbit anti‑Mouse HPSE / Heparanase Antibody (WB) LS‑C662083

Polyclonal Rabbit anti‑Mouse HPSE / Heparanase Antibody (WB) LS‑C662083

Antibody:
HPSE / Heparanase Rabbit anti-Mouse Polyclonal Antibody
Application:
WB
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662083-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HPSE / Heparanase Rabbit anti-Mouse Polyclonal Antibody
Application:
WB
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Heparanase antibody LS-C662083 is an unconjugated rabbit polyclonal antibody to mouse Heparanase (HPSE). Validated for WB.
Target
Mouse HPSE / Heparanase
Synonyms
HPSE | Endo-glucoronidase | Heparanase-1 | Heparanase | HPA | HSE1 | HEP | HPA1 | HPSE1 | HPR1
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived mouse Heparanase 1 recombinant protein (Position: K206-K457). Mouse Heparanase 1 shares 80.2% and 96.4% amino acid (aa) sequence identity with human and rat Heparanase 1, respectively.
Specificity
Expressed in skin, mainly in the stratum granulosum and the first layer of the stratum corneum in the upper part of the epidermis. Also detected in hair follicles and in sebaceous glands. .
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HPSE / Heparanase
Q9Y251 NM_006665 NP_006656.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/17/2024