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Antibodies > HMBS / PBGD > LS-C796862
 
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Catalog Number Size Price
LS-C796862-100 100 µg $539 
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-HMBS mouse monoclonal antibody. heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded Human muscle tissue using anti-HMBS mouse monoclonal antibody. heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-HMBSmouse monoclonal antibody. heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-HMBS mouse monoclonal antibody. heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-HMBS mouse monoclonal antibody. heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-HMBS mouse monoclonal antibody. heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded human tonsil using anti-HMBS clone UMAB144 mouse monoclonal antibody at 1:400 with Polink2 Broad HRP DAB detection kit ; heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Strong cytoplasmic and membraneous staining is seen on tonsil germinal and non-germinal centers. Some weak nuclear stain was also observed.
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-HMBS mouse monoclonal antibody. heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded human prostate cancer using anti-HMBS clone UMAB144 mouse monoclonal antibody at 1:400 with Polink2 Broad HRP DAB detection kit ; heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Weak nuclear staining is seen on tumor cells.
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HMBS mouse monoclonal antibody. heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-HMBS mouse monoclonal antibody. heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
HMBS / PBGD Antibody - Immunofluorescent staining of HeLa cells using anti-HMBS mouse monoclonal antibody green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin. (red), and nuclear with DAPI. (blue). Scale bar, 20µm.
HMBS / PBGD Antibody - Western blot analysis of K562 cell lysate. (35ug) by using anti-HMBS monoclonal antibody.
HMBS / PBGD Antibody - Western blot analysis of KM12 cell lysate. (35ug) by using anti-HMBS monoclonal antibody.
HMBS / PBGD Antibody - Western blot analysis of HT29 cell lysate. (35ug) by using anti-HMBS monoclonal antibody.
HMBS / PBGD Antibody - Western blot of human tissue lysates. (15ug) from 10 different tissues. (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-HMBS mouse monoclonal antibody. heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded Human muscle tissue using anti-HMBS mouse monoclonal antibody. heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-HMBSmouse monoclonal antibody. heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-HMBS mouse monoclonal antibody. heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-HMBS mouse monoclonal antibody. heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-HMBS mouse monoclonal antibody. heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded human tonsil using anti-HMBS clone UMAB144 mouse monoclonal antibody at 1:400 with Polink2 Broad HRP DAB detection kit ; heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Strong cytoplasmic and membraneous staining is seen on tonsil germinal and non-germinal centers. Some weak nuclear stain was also observed.
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-HMBS mouse monoclonal antibody. heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded human prostate cancer using anti-HMBS clone UMAB144 mouse monoclonal antibody at 1:400 with Polink2 Broad HRP DAB detection kit ; heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Weak nuclear staining is seen on tumor cells.
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HMBS mouse monoclonal antibody. heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
HMBS / PBGD Antibody - Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-HMBS mouse monoclonal antibody. heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
HMBS / PBGD Antibody - Immunofluorescent staining of HeLa cells using anti-HMBS mouse monoclonal antibody green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin. (red), and nuclear with DAPI. (blue). Scale bar, 20µm.
HMBS / PBGD Antibody - Western blot analysis of K562 cell lysate. (35ug) by using anti-HMBS monoclonal antibody.
HMBS / PBGD Antibody - Western blot analysis of KM12 cell lysate. (35ug) by using anti-HMBS monoclonal antibody.
HMBS / PBGD Antibody - Western blot analysis of HT29 cell lysate. (35ug) by using anti-HMBS monoclonal antibody.
HMBS / PBGD Antibody - Western blot of human tissue lysates. (15ug) from 10 different tissues. (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.
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Monoclonal Mouse anti‑Human HMBS / PBGD Antibody (clone UMAB144, Carrier‑free, IHC, IF, WB) LS‑C796862

Monoclonal Mouse anti‑Human HMBS / PBGD Antibody (clone UMAB144, Carrier‑free, IHC, IF, WB) LS‑C796862

Submit Review LSBio Guarantee
Antibody:
HMBS / PBGD Mouse anti-Human Monoclonal (Carrier-free) (UMAB144) Antibody
Application:
IHC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:
Unmodified
Price
Catalog Number
$539
LS-C796862-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
HMBS / PBGD Mouse anti-Human Monoclonal (Carrier-free) (UMAB144) Antibody
Application:
IHC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:
Unmodified

Specifications

Description
PBGD antibody LS-C796862 is an unconjugated mouse monoclonal antibody to human PBGD (HMBS). Validated for IF, IHC and WB.
Target
Human HMBS / PBGD
Synonyms
HMBS | Hydroxymethylbilane synthase | PBG-D | PBGD | PORC | Porphyria, acute Chester type | Porphobilinogen deaminase | Pre-uroporphyrinogen synthase | UPS | Uroporphyrinogen I synthetase | Uroporphyrinogen I synthase
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
UMAB144
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Carrier-free. Also available Unmodified.
Immunogen
Full length human recombinant protein of human HMBS (NP_000181) produced in HEK293T cell.
Specificity
The specificity of this antibody has been validated by testing against a high-density Protein Microarray containing more than 17,000 recombinant proteins.
Applications
  • IHC (1:200)
  • Immunofluorescence
  • Western blot (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.3, 8% Trehalose
Reconstitution
Reconstitute with PBS pH 7.3. To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HMBS / PBGD
P08397 NM_000190 NP_000181.2

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Featured Products

HMBS / PBGD Antibody - Western blot analysis of extracts of various cell lines, using HMBS antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.
HMBS / PBGD Antibody LS‑C331681
Species: Human, Mouse
Applications: IHC, Western blot
HMBS / PBGD Antibody - Western blot analysis of HMBS using anti-HMBS antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,Lane 2: human placenta tissue lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human 22RV1 whole cell lysates,Lane 5: human U20S whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMBS antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HMBS at approximately 45KD. The expected band size for HMBS is at 39KD.
HMBS / PBGD Antibody LS‑C662937
Species: Human
Applications: Western blot, ELISA
HMBS / PBGD ELISA Kit
Human HMBS / PBGD (Sandwich ELISA) ELISA Kit - LS-F49603
Reactivity: Human
Range: 0.625-40 ng/ml
HMBS / PBGD ELISA Kit
Mouse HMBS / PBGD (Sandwich ELISA) ELISA Kit - LS-F49606
Reactivity: Mouse
Range: 78-5000 pg/ml
PANX1 / Pannexin 1 ELISA Kit
Human PANX1 / Pannexin 1 (Sandwich ELISA) ELISA Kit - LS-F9953
Reactivity: Human
Range: 0.156-10 ng/ml
LPA / Lysophosphatidic Acid ELISA Kit
LPA / Lysophosphatidic Acid (Competitive EIA) ELISA Kit - LS-F25111
Reactivity:
Range: 123.5-10000 ng/ml
See all HMBS / PBGD Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/1/2024
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