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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HLA‑F Antibody (aa110‑159, FITC) LS‑C452306
HLA-F antibody LS-C452306 is an FITC-conjugated rabbit polyclonal antibody to HLA-F from human, bovine, chimpanzee and other species. Validated for WB.
Catalog
Size
Price
LS-C452306-100
100 µl (1 mg/ml)
$385

Popular HLA-F Products

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Bovine
Applications: Western blot
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Immunofluorescence staining of Hela cells diluted at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Species: Human
Applications: Immunofluorescence, ELISA
Western blot analysis of extracts of A-549 cells, using HLA-F antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
Species: Human, Rat
Applications: Western blot

Product Description

HLA-F antibody LS-C452306 is an FITC-conjugated rabbit polyclonal antibody to HLA-F from human, bovine, chimpanzee and other species. Validated for WB.

Specifications

Target
Human HLA-F
Synonyms
HLA-F, CDA12, HLAF, HLA-CDA12, MHC class I antigen F, HLA F antigen, MHC class Ib antigen, HLA-5.4, Leukocyte antigen F
Host
Rabbit
Reactivity
Human, Chimpanzee, Monkey, Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Fluorescein. Also available Unconjugated or conjugated with Biotin, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
HLA-F antibody was raised against synthetic peptide located between aa110-159 of human HLA-F (P30511, NP_001091949). Percent identity by BLAST analysis: Human, Chimpanzee, Monkey, Bovine (100%).
Epitope
aa110-159
Specificity
Human HLA-F
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS
Storage
Short term: store at 4°C. Long term: add glycerol to 40-50%, aliquot to avoid freeze-thaw cycles, and store at -20°C. Protect from light.
Restrictions
For research use only.
About HLA-F
HLA-F belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. P30511 NM_018950 NP_061823.2


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Images

Western blot

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/12/2018

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