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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C745479-50 50 µg (0.64 mg/ml) $455 
HIST4H4 Antibody - Immunofluorescence of rabbit Anti-Histone H4 [Monomethyl Arg3] Antibody. Tissue: Nonmitotic, prophase, and telophase HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [Monomethyl Arg3] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [Monomethyl Arg3] is nuclear and chromosomal. Staining: Histone H4 [Monomethyl Arg3] is expressed in green, nuclei are counterstained with DAPI (blue).
HIST4H4 Antibody - Western Blot of rabbit Anti-Histone H4 [Monomethyl Arg3] Antibody. Lane 1: NIH-3T3 histone preps. Load: 30 µg per lane. Primary antibody: Histone H4 [Monomethyl Arg3] at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.
HIST4H4 Antibody - Immunofluorescence of rabbit Anti-Histone H4 [Monomethyl Arg3] Antibody. Tissue: Nonmitotic, prophase, and telophase HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [Monomethyl Arg3] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [Monomethyl Arg3] is nuclear and chromosomal. Staining: Histone H4 [Monomethyl Arg3] is expressed in green, nuclei are counterstained with DAPI (blue).
HIST4H4 Antibody - Western Blot of rabbit Anti-Histone H4 [Monomethyl Arg3] Antibody. Lane 1: NIH-3T3 histone preps. Load: 30 µg per lane. Primary antibody: Histone H4 [Monomethyl Arg3] at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.
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HIST4H4 Antibody LS‑C745479

HIST4H4 Antibody LS‑C745479

Antibody:
Rabbit Polyclonal to Human HIST4H4
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$455 
LS-C745479-50
50 µg
0.64 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human HIST4H4
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HIST4H4 antibody LS-C745479 is an unconjugated rabbit polyclonal antibody to HIST4H4 from human, mouse and rat. Validated for IF, IHC and WB.
Target
Human HIST4H4
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Applications
  • IHC (1:50)
  • Immunofluorescence (1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Histone H4 [Monomethyl Arg3] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Arginine 3 of human Histone H4.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% Sodium Azide, 30% Glycerol
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIST4H4

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Requested From: United States
Date Requested: 1/28/2020